Gen-miR-5 derived from Gentianella acuta inhibits PFKP to prevent fibroblast activation and alleviate myocardial fibrosis

Figures

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  FIGURE 1. Gen-miR-5 specifically present in G.acuta might enter the mouse body after exogenous administration to mice (A) Flowchart of High-Throughput Sequencing and Bioinformatics Analysis. (B) Schematic illustration of in vivo imaging of Gen-miR-5 in mice. (C) miRNA was extracted from blood and cardiac tissues and treated with sodium periodate, followed by qRT-PCR to assess the expression levels of Gen-miR-5. Values are expressed as mean ± SEM. *** P < 0.001, **** P < 0.0001 compared to the corresponding control group (n = 4). (D) Gen-miR-5 in cardiac tissue was detected using RNA fluorescence in situ hybridization. Red fluorescence indicates Gen-miR-5 and blue fluorescence marks the nuclei. Scale bar = 50 μm. (E) H&E staining of mouse liver and kidney tissue sections. Scale bar = 2 mm.

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  FIGURE 2. Gen-miR-5 inhibits myocardial fibrosis C57BL/6 mice were subcutaneously infused with Ang II (1.5 mg/kg/day) or an equivalent volume of saline, continuously for 2 weeks, while Gen-miR-5 was administered orally every 2 days (A) H&E staining of cross-sectional slices of cardiac tissue. The white arrow indicates the structural changes and inflammatory infiltration occurring in the cardiac tissue. Scale bar = 20 μm. (B) Masson’s trichrome staining. The white arrow indicates the deposition of collagen fibers. Scale bar = 20 μm. (C) Sirius Red staining. The red arrow indicates the deposition of collagen fibers. Scale bar = 100 μm. (D) The electrocardiogram represents the image. The red arrow indicates the ST-segment elevation. (E) Representative echocardiogram images. (F–I) Ejection fraction (EF), fractional shortening (FS), and left ventricular posterior wall (LVPW) thickness at end-systole and end-diastole. Values are expressed as mean ± SEM. * P < 0.05, *** P < 0.001 compared to the corresponding control group. # P < 0.05, ### P < 0.001 compared to the corresponding Ang II + miR-Ctl group (n = 6).

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  FIGURE 3. Gen-miR-5 inhibits the activation of cardiac fibroblasts (A) After treating cardiac fibroblasts with varying doses of Ang II for 24 h, protein expression levels of Collagen III, Collagen I, and α-SMA were assessed using Western blot (n = 3). (B) Cardiac fibroblasts were treated with Ang II (1 μM) at specified times, and the protein expression levels of Collagen III, Collagen I, and α-SMA were assessed using Western blot (n = 3). (C) Protein expression levels of Collagen III, Collagen I, and α-SMA in cardiac fibroblasts were assessed using Western blot (n = 3). (D) Protein expression levels of Collagen III, Collagen I, and α-SMA in cardiac tissue were measured using Western blot (n = 3). (E) mRNA levels of Collagen III, Collagen I, and α-SMA in cardiac tissue were measured using qRT-PCR (n = 3). Values are expressed as mean ± SEM. * P < 0.05, *** P < 0.001, ## P < 0.01, ### P < 0.001, $ P < 0.05, $$ P < 0.01, $$$ P < 0.001 compared to the corresponding control group.

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  FIGURE 4. Gen-miR-5 inhibits Ang II-induced CF proliferation and migration (A) Protein expression levels of MMP9, MMP2, CyclinD1, and PCNA in cardiac tissue were assessed using Western blot (n = 3). (B) Immunohistochemical staining was used to assess the expression of MMP2 and PCNA in cardiac tissue. Scale bar = 50 μm. (C) Immunofluorescence staining was used to assess the expression of Ki67 in cardiac tissue. Red fluorescence indicates Ki67 and blue fluorescence marks the nuclei. Scale bar = 50 μm. (D) Protein expression levels of MMP9, MMP2, CyclinD1, and PCNA in cardiac fibroblasts were assessed using Western blot (n = 3). (E) Transwell assay used to assess the invasiveness of cardiac fibroblasts. Scale bar = 100 μm. (F) Quantification of cell invasion rate. (G) Wound healing assay to assess cardiac fibroblast migration. Scale bar = 200 μm. (H) Quantification of cell migration rate. Values are expressed as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, # P < 0.05, ## P < 0.01, ### P < 0.001 compared to the corresponding control group.

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  FIGURE 5. Gen-miR-5 can inhibit the expression of PFKP induced by Ang II (A) After treating cardiac fibroblasts with varying doses of Ang II for 24 h, PFKP protein expression levels were assessed using Western blot (n = 3). (B) Cardiac fibroblasts were treated with Ang II (1 μM) at specified times, and PFKP protein expression levels were assessed using Western blot (n = 3). (C) PFKP protein expression levels in cardiac fibroblasts were assessed using Western blot (n = 3). (D) PFKP mRNA levels in cardiac fibroblasts were measured using qRT-PCR (n = 3). (E) Dual-luciferase reporter assay was used to determine the binding interaction between PFKP and Gen-miR-5 in cardiac fibroblasts. The binding sites of Gen-miR-5 on the 3′ UTR of PFKP mRNA are marked in red, while the mutation sites are marked in green. (F) PFKP protein expression levels in cardiac fibroblasts were assessed using Western blot (n = 3). (G) Immunofluorescence staining was used to assess the expression of PFKP in cardiac fibroblasts. Red fluorescence indicates PFKP and blue fluorescence marks the nuclei. Scale bar = 100 μm. Values are expressed as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the corresponding…

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  FIGURE 6. Gen-miR-5 targets PFKP to inhibit the proliferation and migration of CFs (A) PFKP protein expression levels in cardiac fibroblasts were assessed using Western blot (n = 3). (B) PFKP mRNA levels in cardiac fibroblasts were measured using qRT-PCR (n = 3). (C) PFKP protein expression levels in cardiac fibroblasts were assessed using Western blot (n = 3). (D) Protein expression of Collagen III, Collagen I, and α-SMA in cardiac fibroblasts was detected by Western blot (n = 3). (E) Collagen III mRNA levels in cardiac fibroblasts were detected by qRT-PCR (n = 3). (F) Collagen III expression in cardiac fibroblasts was detected by immunofluorescence staining. Red fluorescence is Collagen III and blue fluorescence is the nucleus. (G) PFKP protein expression levels in cardiac fibroblasts were assessed using Western blot (n = 3). (H) Protein expression of Collagen III, Collagen I, and α-SMA in cardiac fibroblasts was detected by Western blot (n = 3). Values are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ##P < 0.01,###P < 0.001 compared to the corresponding control group.

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  FIGURE 7. PFKP synergistically induces CF proliferation and migration in conjunction with lactate (A) Ki67 expression in cardiac fibroblasts was detected by immunofluorescence staining. Red fluorescence is Ki67 and blue fluorescence is nucleus. Scale bar = 100 μm. (B) Transwell detection of myocardial fibroblast invasion. Scale bar = 100 μm. (C) Quantification of cell invasion rate. (D) Cell wound healing assay to detect myocardial fibroblast migration. Scale bar = 200 μm. (E) Quantification of cell migration rate. (F) Changes in lactate levels. (G) Wound healing assay to assess cardiac fibroblast migration. Scale bar = 200 μm. (H) Ki67 expression in cardiac fibroblasts was detected by immunofluorescence staining. Red fluorescence is Ki67 and blue fluorescence is nucleus. Scale bar = 100 μm. (I) Transwell detection of myocardial fibroblast invasion. Scale bar = 100 μm. (J) Quantification of cell invasion rate. (K) Cell wound healing assay to detect myocardial fibroblast migration. Scale bar = 200 μm. (L) Quantification of cell migration rate. Values are expressed as mean ± SEM. ***P < 0.001, ###P < 0.001 compared to the corresponding control group.