Key result
Modulation of the ryanodine receptor using low concentrations of caffeine produced a transient potentiation of systolic Ca2+ to 800% of control, but had no maintained effect on systolic Ca2+.
Why the study?
Does caffeine modulate Ca2+-induced Ca2+ release and affect systolic Ca2+ in rat ventricular myocytes?
Population
Single rat ventricular myocytes
Comparison
Low concentrations of caffeine in reduced… vs Control conditions (no caffeine)
Design
Preclinical
Authors
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Ryanodine receptor modulation yields only transient effects in rat myocytes; leaves open clinical relevance for human systolic Ca2+ handling.
Does caffeine modulate Ca2+-induced Ca2+ release and affect systolic Ca2+ in rat ventricular myocytes?
Modulation of the ryanodine receptor by caffeine has no maintained effect on systolic Ca2+ due to the interdependence of SR Ca2+ content, cytoplasmic Ca2+ buffering, and sarcolemmal Ca2+ fluxes.
Trafford et al. (2000) studied Rat ventricular myocytes. Caffeine vs. Control conditions was evaluated on Systolic [Ca2+]i. Modulation of the ryanodine receptor using low concentrations of caffeine produced a transient potentiation of systolic Ca2+ to 800% of control, but had no maintained effect on systolic Ca2+.
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