Key result
RNA interference-mediated silencing of endogenous xMiRP2 in Xenopus oocytes reduced its expression 5-fold and increased macroscopic HERG current up to 10-fold compared to control siRNA.
Endogenous Xenopus MiRP subunits contribute to the baseline properties of K+ channels like HERG in oocyte expression studies, which could explain expression level- and expression system-dependent variation in K+ channel function.
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May confound HERG studies in oocytes; leaves open MiRP roles in native human cardiac currents.
Anantharam et al. (2003) studied this question. xMiRP2 sequence-specific short interfering RNA (siRNA) vs. Control siRNA oligo was evaluated on Endogenous xMiRP2 expression and macroscopic HERG current. RNA interference-mediated silencing of endogenous xMiRP2 in Xenopus oocytes reduced its expression 5-fold and increased macroscopic HERG current up to 10-fold compared to control siRNA.