What is the clinical evidence from this study?

Population: Acute myocarditis. Intervention: Plasmid vector expressing mouse IL-10 cDNA. Primary outcome: Mast cell density, cardiac histamine concentration, myocardial function, and inflammatory infiltration.

What question did this study set out to answer?

To determine whether intramuscular delivery of an interleukin-10 gene vector protects against acute autoimmune myocarditis by suppressing cardiac mast cell activity.

November 17, 2004European Journal of ImmunologyOpen Access

Inhibition of mast cells by interleukin‐10 gene transfer contributes to protection against acute myocarditis in rats

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Key result

Interleukin-10 gene transfer by electroporation significantly reduced mast cell density and cardiac histamine concentration, and improved myocardial function in rats with acute myocarditis.

Why the study?

Does IL-10 gene transfer improve myocardial function and reduce inflammation in a rat model of acute myocarditis?

Key Points

To determine whether intramuscular delivery of an interleukin-10 gene vector protects against acute autoimmune myocarditis by suppressing cardiac mast cell activity.
Nine-week-old male Lewis rats were immunized with cardiac myosin to induce acute experimental autoimmune myocarditis.
A plasmid vector encoding mouse IL-10 cDNA (800 µg per rat) was delivered three times (days 7, 12, and 17 post-immunization) into the tibialis anterior muscle using in vivo electroporation.
Cardiac tissue was evaluated using toluidine blue-stained ventricular cross-sections to assess mast cell density alongside echocardiographic, hemodynamic, and histological analyses.
Interleukin-10 gene transfer significantly reduced cardiac mast cell density, suppressed mast cell proliferation, prevented degranulation, and decreased myocardial histamine concentration.
Treated animals exhibited reduced heart weight-to-body weight ratios and markedly diminished myocardial inflammatory cell infiltration.
Hemodynamic measurements and echocardiographic indices of cardiac function showed marked functional recovery following IL-10 treatment.

Structured PICO

Does IL-10 gene transfer improve myocardial function and reduce inflammation in a rat model of acute myocarditis?

Population

9-week-old male Lewis rats immunized with cardiac myosin to induce acute myocarditis

Intervention

Plasmid vector expressing mouse IL-10 cDNA (800 mug per rat) transferred three times (7, 12 and 17 days after immunization) into the tibialis anterior muscles by electroporation

Outcome

Mast cell density, cardiac histamine concentration, mast cell growth, and mast cell degranulationsurrogate

IL-10 gene transfer protects against acute myocarditis in a rat model by inhibiting mast cell activity and improving myocardial function.

Authors

SPSuresh S. PalaniyandiHenry Ford Health System KWKenichi WatanabeNiigata University of Pharmacy and Medical and Life Sciences MMMeilei MaNiigata University of Pharmacy and Medical and Life Sciences

Discussion

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Implication

Hypothesis-generating in rat myocarditis; leaves open translation to human disease pending controlled trials.

Cite This Study

Palaniyandi et al. (2004) studied Acute myocarditis. Plasmid vector expressing mouse IL-10 cDNA was evaluated on Mast cell density, cardiac histamine concentration, myocardial function, and inflammatory infiltration. Interleukin-10 gene transfer by electroporation significantly reduced mast cell density and cardiac histamine concentration, and improved myocardial function in rats with acute myocarditis.

synapsesocial.com/papers/6a16637ac1ea77d8cb6236fd https://doi.org/10.1002/eji.200425147

Topics

Myocarditis

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Inhibition of mast cells by interleukin‐10 gene transfer contributes to protection against acute myocarditis in rats

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Topics

Inhibition of mast cells by interleukin‐10 gene transfer contributes to protection against acute myocarditis in rats

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Topics

Also Consider