Vgamma1+ T cells suppress Coxsackievirus B3-induced myocarditis by biasing toward a Th2 response, whereas Vgamma4+ T cells promote myocarditis by biasing toward a Th1 response in mice.
In a murine model of Coxsackievirus B3-induced myocarditis, Vγ1+ T cells suppress cardiac inflammation via Th2 bias, while Vγ4+ T cells promote it via Th1 bias.
Coxsackievirus B3 infections of C57BL/6 mice, which express the MHC class II IA but not IE Ag, results in virus replication in the heart but minimal myocarditis. In contrast, Bl.Tg.Ealpha mice, which are C57BL/6 mice transgenically induced to express IE Ag, develop significant myocarditis upon Coxsackievirus B3 infection. Despite this difference in inflammatory damage, cardiac virus titers are similar between C57BL/6 and Bl.Tg.Ealpha mice. Removing gammadelta T cells from either strain by genetic manipulation (gammadelta knockout(ko)) changes the disease phenotype. C57BL/6 gammadelta ko mice show increased myocarditis. In contrast, Bl.Tg.Ealpha gammadelta ko mice show decreased cardiac inflammation. Flow cytometry revealed a difference in the gammadelta cell subsets in the two strains, with Vgamma1 dominating in C57BL/6 mice, and Vgamma4 predominating Bl.Tg.Ealpha mice. This suggests that these two Vgamma-defined subsets might have different functions. To test this possibility, we used mAb injection to deplete each subset. Mice depleted of Vgamma1 cells showed enhanced myocarditis, whereas those depleted of Vgamma4 cells suppressed myocarditis. Adoptively transfusing enriched Vgamma4(+) cells to the C57BL/6 and Bl.Tg. Ealpha gammadelta ko strains confirmed that the Vgamma4 subset promoted myocarditis. Th subset analysis suggests that Vgamma1(+) cells biased the CD4(+) T cells to a dominant Th2 cell response, whereas Vgamma4(+) cells biased CD4(+) T cells toward a dominant Th1 cell response.
Huber et al. (Sun,) conducted a other in Coxsackievirus B3-induced myocarditis. Depletion or adoptive transfer of Vgamma1+ or Vgamma4+ T cells vs. Control mice was evaluated on Myocarditis severity and cardiac inflammation. Vgamma1+ T cells suppress Coxsackievirus B3-induced myocarditis by biasing toward a Th2 response, whereas Vgamma4+ T cells promote myocarditis by biasing toward a Th1 response in mice.