What does this research mean for the field?

The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method provides a highly reliable and efficient way to detect sequence variations, with detection rates depending primarily on fragment length rather than the location or type of substitution. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

December 1, 1998Electrophoresis

Polymerase chain reaction—single strand conformation polymorphism or how to detect reliably and efficiently each sequence variation in many samples and many genes

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Population

55 PCR systems, including a total of 145 fragments from several genes thought to be involved in the…

Design

Other

Authors

SJSilke Jaeckel JEJörg T. EpplenAmedes Genetics (Germany)MKMarion Kauth

Discussion

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Implication

May aid variant screening where sequencing access is limited; leaves open validation against NGS in cardiovascular cohorts.

Structured PICO

Population

55 PCR systems, including a total of 145 fragments from several genes thought to be involved in the pathogenesis of multifactorial autoimmune diseases like multiple sclerosis

Intervention

Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis

Outcome

Detection rate of mutations, polymorphisms and sequence variants (MPSV)

PCR-SSCP is a reliable and efficient method for detecting sequence variations across multiple genes and samples.

Cite This Study

Jaeckel et al. (1998) studied this question.

synapsesocial.com/papers/6a17cb7dcf49e78c48b475cf https://doi.org/10.1002/elps.1150191802

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Polymerase chain reaction—single strand conformation polymorphism or how to detect reliably and efficiently each sequence variation in many samples and many genes

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Polymerase chain reaction—single strand conformation polymorphism or how to detect reliably and efficiently each sequence variation in many samples and many genes

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider