Expression of cellular oncogenes in the myocardium during the developmental stage and pressure-overloaded hypertrophy of the rat heart.
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Discussion
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Implication
Experimental study reveals stage-specific cellular oncogene activation during development and pressure overload in rats, suggesting shared pathways between hypertrophy and cell proliferation.
Key Points
- To determine the role and expression patterns of cellular oncogenes during normal cardiac development and pressure-overload cardiac hypertrophy in vivo.
- Assessed in vivo expression of eight cellular oncogenes (myc, fos, Ha-ras, src, erbA, erbB, sis, myb) in rat myocardium across embryonic, fetal, and postnatal developmental stages.
- Induced pressure-overloaded hypertrophy via surgical aortic constriction and measured myocardial oncogene expression at intervals up to 48 hours using Northern blot analysis.
- Aortic constriction induced rapid increases in c-fos (detectable at 30 minutes) and c-myc (at 2 hours), both peaking at 8 hours and returning to baseline by 48 hours, while c-Ha-ras showed a gradual increase.
- Developmental expression was stage-specific: c-myc was detectable only during embryonic life, c-fos was absent prenatally and peaked in 200-day-old adults, and c-Ha-ras remained constant throughout development.
Cite This Study
Komuro et al. (1988) studied this question.