Key result
Antisense oligonucleotides targeting the alpha-subunit of ENaC significantly decreased the density of nonselective cation channels in rat alveolar type II cells, unlike beta- or gamma-subunit targets.
Population
Rat alveolar type II (ATII) cells in primary culture
Design
Preclinical
Authors
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Alpha ENaC targeting reduces rat alveolar cation channels; challenges multi-subunit consensus and leaves open human translation.
The alpha-subunit of ENaC alone or in combination with a non-beta/gamma protein is the major component of lung alveolar epithelial cation channels.
Jain et al. (1999) studied this question. Antisense oligonucleotides targeting ENaC subunits (alpha, beta, and gamma) was evaluated on Density of the nonselective cation channel observed in apical membrane patches. Antisense oligonucleotides targeting the alpha-subunit of ENaC significantly decreased the density of nonselective cation channels in rat alveolar type II cells, unlike beta- or gamma-subunit targets.
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