October 1, 1996AJP Cell Physiology

Cell-specific expression of amiloride-sensitive, Na(+)-conducting ion channels in the kidney

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Population

Mouse kidney cell lines and/or dissected nephron segments

Design

Preclinical

Authors

FCF. CiampolilloDartmouth College DMD. E. McCoyDartmouth College RGR. B. GreenDartmouth College

Discussion

Member takes

Implication

Preclinical rodent data offer no immediate clinical guidance; leaves open coordinated cGMP-ENaC regulation of renal sodium handling in humans.

Structured PICO

Population

Mouse kidney cell lines and/or dissected nephron segments

Intervention

In situ reverse transcription-polymerase chain reaction (RT-PCR) hybridization, solution-phase RT-PCR, and Western blot analysis

Outcome

Expression of cGMP-gated cation channel and ENaC mRNA and protein in specific nephron segmentssurrogate

The coexpression of ENaC and cGMP-gated cation channels in multiple nephron segments suggests both contribute to renal sodium absorption and may be regulated by cGMP-modulating hormones.

Cite This Study

Ciampolillo et al. (1996) studied this question.

synapsesocial.com/papers/6a912b09be68d22e6965e1d4 https://doi.org/10.1152/ajpcell.1996.271.4.c1303

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Implication

Preclinical rodent data offer no immediate clinical guidance; leaves open coordinated cGMP-ENaC regulation of renal sodium handling in humans.