What does this research mean for the field?

Titin is present in punctate and diffuse forms in the cytoplasm of cardiac myocytes during premyofibril stages, independent of myosin and actin, and these titin spots are subsequently incorporated into newly arising myofibrils. Novelty: ClaimNovelty.CONTRADICTORY. Consensus alignment: ConsensusAlignment.CHALLENGES_CONSENSUS.

December 1, 1987The Journal of Cell BiologyOpen Access

Immunocytochemical studies of cardiac myofibrillogenesis in early chick embryos. I. Presence of immunofluorescent titin spots in premyofibril stages.

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Population

4-12 somite stage chick embryos

Design

Preclinical

Authors

KTK. T. TokuyasuKurume University PMPamela MaherSalk Institute for Biological Studies

Discussion

Member takes

Implication

Challenges prior titin-myosin linkage models in myofibrillogenesis; extends early scaffolding role but animal data leave clinical translation open.

Structured PICO

Population

4-12 somite stage chick embryos

Intervention

Immunofluorescent labeling with antibodies to titin, myosin, or actin or with NBD-phallacidin for F-actin after treatment with hyaluronidase and nonionic detergent

Outcome

Localization and distribution of titin, myosin, and actin during cardiac myofibrillogenesis

Titin is present in premyofibril stages of cardiac myocytes independently of myosin and actin organization, suggesting it acts as an early scaffolding protein during myofibrillogenesis.

Cite This Study

Tokuyasu et al. (1987) studied this question.

synapsesocial.com/papers/6a18aef1673175fe754ac72a https://doi.org/10.1083/jcb.105.6.2781

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Implication

Challenges prior titin-myosin linkage models in myofibrillogenesis; extends early scaffolding role but animal data leave clinical translation open.

Immunocytochemical studies of cardiac myofibrillogenesis in early chick embryos. I. Presence of immunofluorescent titin spots in premyofibril stages.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Immunocytochemical studies of cardiac myofibrillogenesis in early chick embryos. I. Presence of immunofluorescent titin spots in premyofibril stages.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider