What is the clinical evidence from this study?

Study design: Other. Population: Healthy wild-type rat cardiomyocytes (n=2043). Intervention: Atrial cardiomyocytes vs. Ventricular cardiomyocytes. Primary outcome: Fractional shortening (%) (p=<0.001).

What does this research mean for the field?

Atrial cardiomyocytes demonstrate a twofold lower fractional shortening and slower contraction and relaxation kinetics compared to ventricular cardiomyocytes, which aligns with organ-level atrioventricular differences but only partially explains interventricular differences. Novelty: ClaimNovelty.CONFIRMATORY. Consensus alignment: ConsensusAlignment.SUPPORTS_CONSENSUS.

July 21, 2020Frontiers in PhysiologyOpen Access

Large-Scale Contractility Measurements Reveal Large Atrioventricular and Subtle Interventricular Differences in Cultured Unloaded Rat Cardiomyocytes

Q: What are the key findings of this study?

Atrial cardiomyocytes demonstrated a twofold lower fractional shortening (4.3% vs 8.4%) and slower contraction and relaxation kinetics compared to ventricular cardiomyocytes.

Q: What does this research mean for the field?

Atrial cardiomyocytes demonstrate a twofold lower fractional shortening and slower contraction and relaxation kinetics compared to ventricular cardiomyocytes, which aligns with organ-level atrioventricular differences but only partially explains interventricular differences. Novelty: ClaimNovelty.CONFIRMATORY. Consensus alignment: ConsensusAlignment.SUPPORTS_CONSENSUS.

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Key result

Atrial cardiomyocytes demonstrated a twofold lower fractional shortening (4.3% vs 8.4%) and slower contraction and relaxation kinetics compared to ventricular cardiomyocytes.

Why the study?

It is incompletely understood whether hemodynamic differences between heart chambers can be explained by contractile differences at the level of individual cardiomyocytes.

Population

2,043 cardiomyocytes from atria, RV, LV, and IVS of five healthy wild-type rats

Comparison

Cardiomyocytes from atria vs RV vs LV vs IVS

Authors

ENEdgar E. NolletAmsterdam Neuroscience EMEmmy M. Manders MGMax GoebelAmsterdam Neuroscience

Discussion

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Implication

Atrial-ventricular myocyte differences should not yet change practice; leaves open chamber-specific roles in atrial disease models.

PICO

Population

Healthy wild-type rat cardiomyocytes (n=2,043)

Intervention / Comparator

Atrial cardiomyocytes vs Ventricular cardiomyocytes

Primary Outcome

Fractional shortening (%), p=<0.001

Main Result

Absolute Event Rate: 4.3% vs 8.4%

p-value: p=<0.001

Limitations

Combined cardiomyocytes from the left and right atrium, obscuring potential differences between these regions.
Measurements were performed after overnight culture, during which regional contractile differences may become less pronounced compared to directly after isolation.
Measurements were performed under unloaded conditions, which may not fully reflect in vivo tissue contraction.

Cite This Study

Nollet et al. (2020) studied Healthy wild-type rat cardiomyocytes (n=2,043). Atrial cardiomyocytes vs. Ventricular cardiomyocytes was evaluated on Fractional shortening (%) (p=<0.001). Atrial cardiomyocytes demonstrated a twofold lower fractional shortening (4.3% vs 8.4%) and slower contraction and relaxation kinetics compared to ventricular cardiomyocytes.

synapsesocial.com/papers/6a18b3088dcaf40f45cfa736 https://doi.org/10.3389/fphys.2020.00815

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Large-Scale Contractility Measurements Reveal Large Atrioventricular and Subtle Interventricular Differences in Cultured Unloaded Rat Cardiomyocytes

Discussion

Member takes

Implication

PICO

Main Result

Limitations

Cite This Study

Large-Scale Contractility Measurements Reveal Large Atrioventricular and Subtle Interventricular Differences in Cultured Unloaded Rat Cardiomyocytes

Discussion

Member takes

Implication

PICO

Main Result

Limitations

Cite This Study

Also Consider

Also Consider