What question did this study set out to answer?

To determine how dynamic endothelial cell-cell junctions interact with stress fibres and maintain mechanical connections between neighbouring cells.

February 2, 2010BMC BiologyOpen Access

Adherens junctions connect stress fibres between adjacent endothelial cells

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Authors

JMJaime MillánCentro de Biología Molecular Severo Ochoa RCRobert J. CainUniversity of Edinburgh NRNatalia Reglero-RealHospital Universitario de La Princesa

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Implication

Experimental study reveals that discontinuous adherens junctions link stress fibres across adjacent endothelial cells, indicating a focal adhesion-independent mechanism for vascular integrity.

Key Points

To determine how dynamic endothelial cell-cell junctions interact with stress fibres and maintain mechanical connections between neighbouring cells.
Examined junctional morphology and actin cytoskeletal architecture in confluent endothelial cell cultures treated with tumour necrosis factor (TNF)-alpha.
Used RNA interference to knock down vascular endothelial (VE)-cadherin or zonula occludens-1 and evaluated effects on junctional stress fibre attachments and focal adhesions.
Discontinuous adherens junctions containing VE-cadherin and catenin complexes directly bind stress fibres, physically connecting neighbouring endothelial cells independently of focal adhesions.
Stimulation with TNF-alpha promotes the formation of discontinuous adherens junctions alongside increased stress fibre assembly.
RNAi depletion of VE-cadherin, but not zonula occludens-1, disrupts stress fibre anchorage to junctions, increases focal adhesions, and reorganizes actin cables.

Cite This Study

Millán et al. (2010) studied this question.

synapsesocial.com/papers/6a18d93ec9d74cf65281fb05 https://doi.org/10.1186/1741-7007-8-11

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Implication

Experimental study reveals that discontinuous adherens junctions link stress fibres across adjacent endothelial cells, indicating a focal adhesion-independent mechanism for vascular integrity.

Adherens junctions connect stress fibres between adjacent endothelial cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Adherens junctions connect stress fibres between adjacent endothelial cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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