Mechanism of the association of HDL3 with endothelial cells, smooth muscle cells, and fibroblasts. Evidence against the role of specific ligand and receptor proteins.
Population
Porcine aortic endothelial cells, rabbit aortic smooth muscle cells, and normal human skin fibroblasts
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Challenges receptor-mediated HDL3 binding models; hypothesis-generating for lipid interactions, needs in vivo confirmation.
Structured PICO
P
Population
Porcine aortic endothelial cells, rabbit aortic smooth muscle cells, and normal human skin fibroblasts
I
Intervention
125I-HDL3 and various competitors (trypsin-digested HDL3, synthetic phospholipid-cholesterol discs, phospholipid-cholesterol vesicles)
O
Outcome
Cellular association and competitive displacement of 125I-HDL3surrogate
HDL3 association with cells is mediated by lipid-lipid interactions rather than specific ligand-receptor proteins.
Cite This Study
Tabas et al. (1984) studied this question.