What question did this study set out to answer?

To investigate how lipid removal and subsequent lipid reconstitution alter the secondary and tertiary conformations of human plasma high-density apolipoproteins.

April 1, 1972Journal of Biological ChemistryOpen Access

The Influence of Lipid on the Conformation of Human Plasma High Density Apolipoproteins

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To investigate how lipid removal and subsequent lipid reconstitution alter the secondary and tertiary conformations of human plasma high-density apolipoproteins.
Measured secondary and tertiary structural changes of native, delipidated, and reconstituted apoHDL, apoA-I, and apoA-II using far- and near-ultraviolet circular dichroism spectroscopy.
Isolated individual apoproteins (apoA-I and apoA-II) using urea chromatography to assess refolding properties upon urea removal.
Reconstituted delipidated apolipoproteins with phosphatidylcholine alone or in combination with cholesteryl oleate/esters.
Native HDL contained approximately 70% alpha-helical, 5% to 15% beta, and 15% to 20% disordered structure, whereas delipidation reduced helical content by about 20% with a corresponding rise in disordered structure.
Reconstitution of apoA-I, apoA-II, and apoHDL with phosphatidylcholine and cholesteryl ester restored 110%, 87%, and 100% of parental helical structure, respectively, whereas phosphatidylcholine alone restored 50% to 70% of this increase.
Near-ultraviolet circular dichroism spectra confirmed that 20% to 30% of amino acid residues participate in a reversible helix-disordered transition dependent on lipid binding.

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Implication

In vitro spectroscopic analysis shows lipid binding restores native helical conformation in human apolipoproteins, highlighting the requirement of both phospholipids and neutral lipids.

Cite This Study

Lux et al. (1972) studied this question.

synapsesocial.com/papers/6a18e92a8dcaf40f45cfe6d1 https://doi.org/10.1016/s0021-9258(19)45468-9

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Implication

In vitro spectroscopic analysis shows lipid binding restores native helical conformation in human apolipoproteins, highlighting the requirement of both phospholipids and neutral lipids.

The Influence of Lipid on the Conformation of Human Plasma High Density Apolipoproteins

Key Points

Discussion

Implication

Cite This Study

The Influence of Lipid on the Conformation of Human Plasma High Density Apolipoproteins

Key Points

Discussion

Implication

Cite This Study

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