What does this research mean for the field?

In smooth muscle cells, cyclic AMP and cyclic GMP enhance intracellular calcium storage, whereas G-protein activation by GTP gamma S depletes these calcium stores. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

July 1, 1989British Journal of PharmacologyOpen Access

Actions of guanine nucleotides and cyclic nucleotides on calcium stores in single patch‐clamped smooth muscle cells from rabbit portal vein

View Full Paper

Ask AI

Bookmark

Structured PICO

Population

Single smooth muscle cells obtained from the rabbit portal vein by enzymic digestion

Intervention

Application of guanine nucleotides (GTP gamma S, GDP beta S), cyclic nucleotides (cyclic AMP, cyclic GMP, 8-bromo cyclic AMP, 8-bromo cyclic GMP), carbachol, caffeine, noradrenaline, and ryanodine

Outcome

Spontaneous outward currents (STOCs) and outward currents resulting from calcium store discharge measured by whole-cell patch clamp techniquesurrogate

In rabbit portal vein smooth muscle cells, cyclic GMP and cyclic AMP enhance calcium storage while GTP gamma S depletes it, suggesting a G-protein step in noradrenaline-evoked calcium store release.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Should not alter vascular therapies; hypothesis-generating for G-protein modulation of calcium stores in smooth muscle.

Cite This Study

Komori et al. (1989) studied this question.

synapsesocial.com/papers/6a198a468ef0a4da5e8dfa69 https://doi.org/10.1111/j.1476-5381.1989.tb12039.x

View Full Paper

Ask AI

Bookmark

Actions of guanine nucleotides and cyclic nucleotides on calcium stores in single patch‐clamped smooth muscle cells from rabbit portal vein

Structured PICO

Discussion

Implication

Cite This Study

Actions of guanine nucleotides and cyclic nucleotides on calcium stores in single patch‐clamped smooth muscle cells from rabbit portal vein

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider