Adenosine A2A and A2B Receptor Substantially Attenuate Ischemia/Reperfusion Injury in Septic rat Hearts

Figures

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  Fig. 1. (a) Protocol 1; (b) protocol 2; CLP = cecal ligation and puncture

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  Fig. 2. (a) AdorA2a and AdorA2b mRNA expression in the left atrium (LA), right atrium (RA), right ventricle (RV), and left ventricle (LV) in SHAM animals. *p < 0.05 ventricle vs. atrium. (b) AdorA2a mRNA expression in the right (RV) and left ventricle (LV) in sham- or CLP-treated animal with or without ischemia (I). (c) AdorA2b mRNA expression in the right (RV) and left ventricle (LV) in sham- or CLP-treated animal with or without ischemia (I). Data are means ± SEM of 5 animals per group. *p < 0.05 vs. sham-treated animals without ischemia (I)

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  Fig. 3. Relative Optical Density (OD) from immunoblots of AdorA2a and AdorA2b of left ventricular tissue 24 h after CLP. Values are given as fold of sham-treated animals. Values are related to signals that were obtained for β-actin. Data are means ± SEM of 3 animals per group. *p < 0.05 vs. sham-treated animals

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  Fig. 4. Pre- and post-I/R left ventricular pressure of CLP, CLP+ SCH58261 + MRS1706, SHAM and SHAM+ SCH58261 + MRS1706. Pre-I/R: *p < 0.05 SHAM vs. CLP and CLP + SCH58261 + MRS1706; # p < 0.05 SHAM+ SCH58261 + MRS1706 vs. CLP and CLP + SCH58261 + MRS1706. Post-I/R vs. Pre-I/R: § p < 0.05 for CLP + SCH58261 + MRS1706, $ p < 0.05 for SHAM and &p < 0.05 for SHAM + SCH58261 + MRS1706. The data are presented as the means ± SEM of 10 animals per group

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  Fig. 5. Increase of coronary flow (CF) after administration of adenosine before and after I/R. *p < 0.05 before I/R and # p < 0.05 after I/R for baseline vs. SHAM, SHAM + SCH58261 + MRS1706, CLP, or CLP+ SCH58261 + MRS1706. The data are presented as the means ± SEM of 10 animals per group

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  Fig. 6. Lactate, CK-MB, and TNI in post-I/R effluat of SHAM, CLP, and CLP + SCH58261 + MRS1706. * and # p < 0.05 SHAM and CLP vs. CLP + SCH58261 + MRS1706, respectively. $ p < 0.05 SHAM vs. CLP. The data are presented as the means ± SEM of 10 animals per group