April 24, 2019PLoS ONEOpen Access

Hypoxia induced hERG trafficking defect linked to cell cycle arrest in SH-SY5Y cells

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Why the study?

Hypoxia alters the gating properties of hERG currents in SH-SY5Y neuroblastoma cells, but the underlying molecular mechanisms and physiological significance were not fully understood.

Population

SH-SY5Y neuroblastoma cells

Comparison

Hypoxia vs normoxia or rescue treatments

Design

Preclinical in vitro experimental study

Authors

VRVaddi Damodara ReddyUniversity of Zululand LPLin PiaoUniversity of Chicago SKShakil A. KhanJohns Hopkins University

Discussion

Member takes

Implication

Hypoxia effects on hERG may suppress neuroblastoma proliferation; hypothesis-generating in cell models and requires in vivo human validation.

Structured PICO

Population

SH-SY5Y neuroblastoma cells and HEK293 cells stably expressing the α subunit of the hERG channel

Intervention

Continuous hypoxia (1.5% O2) for 2 and 4 days, with or without antioxidant (MnTmPyP) or hERG blocker (E-4031)

Comparator

Normoxia

Outcome

hERG protein expression (surface vs ER), hERG K+ current density, and cell proliferation/cell cycle progressionsurrogate

Hypoxia-induced oxidative stress impairs hERG channel trafficking and decreases K+ currents, leading to cell cycle arrest in neuroblastoma cells.

Cite This Study

Reddy et al. (2019) studied this question.

synapsesocial.com/papers/6a19b2e59289b0a87eea67d9 https://doi.org/10.1371/journal.pone.0215905

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Implication

Hypoxia effects on hERG may suppress neuroblastoma proliferation; hypothesis-generating in cell models and requires in vivo human validation.

Hypoxia induced hERG trafficking defect linked to cell cycle arrest in SH-SY5Y cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Hypoxia induced hERG trafficking defect linked to cell cycle arrest in SH-SY5Y cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider