What does this research mean for the field?

Nerve growth factor (NGF) post-transcriptionally increases the abundance of the Kv2.1 potassium channel and regulates its spatial distribution, localizing it to neuritic growth cones in differentiating PC12 cells. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

Determine the impact of nerve growth factor (NGF) on the protein abundance, steady-state mRNA levels, and subcellular distribution of the delayed rectifier K+ channel Kv2.1 in PC12 cells.

December 15, 1993The Journal of Cell BiologyOpen Access

Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells.

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Why the study?

Does nerve growth factor treatment regulate the abundance and distribution of Kv2.1 K+ channels in PC12 cells?

Key Points

Determine the impact of nerve growth factor (NGF) on the protein abundance, steady-state mRNA levels, and subcellular distribution of the delayed rectifier K+ channel Kv2.1 in PC12 cells.
Treated PC12 cells with continuous NGF over time courses ranging from 12 hours to 6 days.
Quantified Kv2.1 polypeptide with anti-Kv2.1 immunoblots and assessed Kv2.1 mRNA expression via RNase protection assays.
Mapped spatial distribution of Kv2.1 protein using immunofluorescence in undifferentiated and differentiated PC12 cells.
NGF treatment stimulated a fourfold increase in membrane-associated 132 kD Kv2.1 polypeptide within 12 hours, which persisted for at least 6 days.
RNase protection assays revealed that the increase in Kv2.1 protein levels occurred without an increase in steady-state Kv2.1 mRNA.
Kv2.1 localized to cell-cell and cell-substratum contacts in undifferentiated cells, but concentrated predominantly in neuritic growth cones following NGF-induced differentiation.

Structured PICO

Does nerve growth factor treatment regulate the abundance and distribution of Kv2.1 K+ channels in PC12 cells?

Population

PC12 cells

Intervention

Nerve growth factor (NGF) treatment

Comparator

Untreated PC12 cells

Outcome

Expression levels and distribution of Kv2.1 polypeptidesurrogate

NGF regulates the abundance and distribution of Kv2.1 potassium channels in PC12 cells, providing a model for studying selective channel expression in neuritic endings.

Discussion

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Implication

Hypothesis-generating in PC12 models; leaves open in vivo or clinical relevance of post-transcriptional Kv2.1 control.

Cite This Study

Sharma et al. (1993) studied this question.

synapsesocial.com/papers/6a19db114b45427442ead11b https://doi.org/10.1083/jcb.123.6.1835

View Full Paper

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Bookmark

Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells.

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells.

Key Points

Structured PICO

Discussion

Implication

Cite This Study

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