What question did this study set out to answer?

Determine whether PC12 cells possess caveolae microdomains and evaluate if the high-affinity receptor TrkA localizes within them to mediate nerve growth factor signaling.

December 1, 2000Journal of Biological ChemistryOpen Access

PC12 Cells Have Caveolae That Contain TrkA

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Determine whether PC12 cells possess caveolae microdomains and evaluate if the high-affinity receptor TrkA localizes within them to mediate nerve growth factor signaling.
Detected caveolin-1 and membrane caveolae in PC12 cells using Western blot, confocal immunomicroscopy, and rapid-freeze deep-etching electron microscopy with immunogold labeling.
Assessed signaling protein cofractionation and measured MAPK phosphorylation following caveolae disruption via cholesterol-binding drugs (filipin and cyclodextrin).
Visualized plasma membrane caveolae containing the NGF receptor TrkA, which cofractionated with caveolin, Shc, and Ras.
Cholesterol extraction with filipin or cyclodextrin elevated basal MAPK phosphorylation and selectively blocked NGF-induced MAPK phosphorylation without altering TrkA autophosphorylation or EGF signaling.

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Implication

In vitro study demonstrates that TrkA localizes to membrane caveolae in PC12 cells, indicating that cholesterol-rich microdomains mediate downstream nerve growth factor signaling.

Cite This Study

Peiró et al. (2000) studied this question.

synapsesocial.com/papers/6a1a859149c3147e1045b930 https://doi.org/10.1074/jbc.m000487200

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Implication

In vitro study demonstrates that TrkA localizes to membrane caveolae in PC12 cells, indicating that cholesterol-rich microdomains mediate downstream nerve growth factor signaling.

PC12 Cells Have Caveolae That Contain TrkA

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Discussion

Implication

Cite This Study

PC12 Cells Have Caveolae That Contain TrkA

Key Points

Discussion

Implication

Cite This Study

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