What does this research mean for the field?

The TGT sequence at the 5' boundary of the pyrimidine-rich region upstream of the 3' splice site in the adenovirus 2 E3 transcription unit is essential for splicing, whereas the primary branchpoint site is dispensable due to the use of a surrogate. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

September 1, 1985Molecular and Cellular Biology

Virus Deletion Mutants That Affect a 3′ Splice Site in the E3 Transcription Unit of Adenovirus 2

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Population

Adenovirus 2 E3 transcription unit

Design

Preclinical

Authors

BBBheem M. BhatPfizer (United States)HBHelen BradyWashington University in St. Louis WWWilliam S.M. WoldSaint Louis University

Discussion

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Implication

No immediate clinical implications from this viral model; leaves open TGT conservation in mammalian splicing.

Structured PICO

Population

Adenovirus 2 E3 transcription unit

Intervention

Virus deletion mutants near a 3' splice site at nucleotide 2157 (dl719, dl717, dl712, dl716, dl714)

Outcome

Splicing activity at the 2157 3' splice site in vivo by nuclease-gel analysis of steady-state cytoplasmic mRNAsurrogate

The TGT sequence at the 5' boundary of the pyrimidine-rich region is required for splicing at the nucleotide 2157 3' splice site in the E3 transcription unit of adenovirus 2.

Cite This Study

Bhat et al. (1985) studied this question.

synapsesocial.com/papers/6a1a48321fae7897603accef https://doi.org/10.1128/mcb.5.9.2405-2413.1985

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Virus Deletion Mutants That Affect a 3′ Splice Site in the E3 Transcription Unit of Adenovirus 2

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Virus Deletion Mutants That Affect a 3′ Splice Site in the E3 Transcription Unit of Adenovirus 2

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider