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November 14, 2025PLoS ONEOpen Access

Breathing mode selectively modulates brain-wide functional connectivity

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Why the study?

How different breathing modes, specifically nasal versus oral, affect frequency-specific large-scale neural connectivity in humans remains unexplored.

Does nasal breathing compared to oral breathing modulate functional brain connectivity in healthy males?

Population

20 healthy male participants

Comparison

Nasal vs oral breathing

Design

Resting-state fMRI study

Key result

Nasal breathing was associated with greater functional connectivity within higher-order brain networks, whereas oral breathing increased connectivity centered on the brainstem (p-FDR < 0.05).

Authors

SMSadeq MohammadiGHGholam‐Ali Hossein‐ZadehMRMohammad Reza Raoufy

Discussion

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Member takes

Overview

Does not support changing breathing practices; leaves open causal effects on cognition or disease.

Study Design

Type

Observational (n=20)

Structured PICO

Does nasal breathing compared to oral breathing modulate functional brain connectivity in healthy males?

P
Population
20 healthy male participants
I
Intervention
Nasal breathing
C
Comparator
Oral breathing
O
Outcome
Functional brain connectivity (blood oxygenation level-dependent BOLD fluctuations in the intermediate frequency band of 0.1-0.2 Hz)surrogate

Main Result

p-value: p=<0.05

Nasal and oral breathing modes engage distinct large-scale brain networks, suggesting respiration mode selectively modulates brain-wide functional connectivity.

Limitations

  • Translational implications require validation in future experimental studies

Cite This Study

Mohammadi et al. (2025) conducted an observational in Healthy (n=20). Nasal breathing vs. Oral breathing was evaluated on Functional brain connectivity (BOLD fluctuations in 0.1-0.2 Hz band) (p=<0.05). Nasal breathing was associated with greater functional connectivity within higher-order brain networks, whereas oral breathing increased connectivity centered on the brainstem (p-FDR < 0.05).

synapsesocial.com/papers/6a1b22f65853c704fce57bdchttps://doi.org/10.1371/journal.pone.0334165
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