The study generated 60 monoclonal antibodies specific to African swine fever virus proteins, aiding in the characterization of viral antigens and their subcellular localization.
Supplies monoclonal reagents for ASFV antigen studies; leaves open diagnostic or vaccine applications pending validation.
We have obtained 60 stable hybridomas which produced immunoglobulins that recognized 12 proteins from African swine fever virus particles and African swine fever virus-infected cells. Most of the monoclonal antibodies were specific for the three major structural proteins p150, p72, and p12. The specificity of some monoclonal antibodies for the structural proteins p150 and p37 and the nonstructural proteins p220 and p60 indicated that proteins p150 and p220 are antigenically related to proteins p37 and p60. The association of some viral antigens to specific subcellular components was determined by immunofluorescence and analysis of the binding of monoclonal antibodies to infected cells. A host protein (p24) seemed to be associated with the virus particles.
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Sanz et al. (1985) studied this question.
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