What does this research mean for the field?

Crossbridge flexibility is a key determinant of crossbridge cycling rates, and C protein phosphorylation regulates actomyosin ATPase activity by modifying the position and flexibility of these crossbridges in a myosin heavy chain isoform-dependent manner. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

July 13, 1998Circulation ResearchOpen Access

Relation Between Crossbridge Structure and Actomyosin ATPase Activity in Rat Heart

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Population

Thick filaments isolated from rat ventricles with either the alpha or beta isoform of myosin heavy chain (MHC)

Comparison

Phosphorylation of C protein by protein kinase A vs Unphosphorylated state / beta-MHC filaments

Design

Preclinical

Authors

AWAndrea S. WeisbergNational Institutes of Health SWSaul WinegradRutgers, The State University of New Jersey

Discussion

Member takes

Implication

No immediate clinical implications; hypothesis-generating for myosin isoform-specific myofilament regulation.

Structured PICO

Population

Thick filaments isolated from rat ventricles with either the alpha or beta isoform of myosin heavy chain (MHC)

Intervention

Phosphorylation of C protein by protein kinase A

Comparator

Unphosphorylated state / beta-MHC filaments

Outcome

Crossbridge structure (extension, orientation, degree of order, flexibility) and rate of ATP hydrolysis by actomyosinsurrogate

Phosphorylation of C protein regulates crossbridge structure and flexibility in a myosin isoform-specific manner, which correlates with actomyosin ATPase activity and crossbridge cycling kinetics.

Cite This Study

Weisberg et al. (1998) studied this question.

synapsesocial.com/papers/6a1be653ea84844e355f261e https://doi.org/10.1161/01.res.83.1.60

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Relation Between Crossbridge Structure and Actomyosin ATPase Activity in Rat Heart

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Relation Between Crossbridge Structure and Actomyosin ATPase Activity in Rat Heart

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider