What does this research mean for the field?

A Mg2+-dependent tropomyosin kinase from chick embryos specifically phosphorylates skeletal muscle alpha-tropomyosin at serine 283, with significantly lower activity toward beta-tropomyosin and no activity toward smooth muscle tropomyosin. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

May 1, 1984Journal of Biological ChemistryOpen Access

In vitro phosphorylation of tropomyosin by a kinase from chicken embryo.

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Population

In vitro model using tropomyosin kinase partially purified from the leg muscle of 11-day-old chick embryos…

Design

Preclinical

Authors

KMKyle MontgomerySouthern Illinois University School of Medicine AMAlan S. MakQueen's University

Discussion

Member takes

Implication

Differential alpha- versus beta-tropomyosin phosphorylation at Ser283 may influence filament dynamics; leaves open relevance to cardiac sarcomere regulation.

Structured PICO

Population

In vitro model using tropomyosin kinase partially purified from the leg muscle of 11-day-old chick embryos, and tropomyosin from rabbit and chicken skeletal muscle and chicken gizzard.

Intervention

In vitro phosphorylation assay evaluating the effects of Mg2+, Ca2+, cyclic AMP, and KCl concentration.

Outcome

Phosphorylation rate of tropomyosin subunits and identification of the specific phosphorylation site.surrogate

This basic science study identifies serine 283 as the specific in vitro phosphorylation site of skeletal muscle alpha-tropomyosin by a kinase from chick embryos.

Cite This Study

Montgomery et al. (1984) studied this question.

synapsesocial.com/papers/6a1bf2fc27b545b111a93f00 https://doi.org/10.1016/s0021-9258(18)91049-5

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Implication

Differential alpha- versus beta-tropomyosin phosphorylation at Ser283 may influence filament dynamics; leaves open relevance to cardiac sarcomere regulation.

In vitro phosphorylation of tropomyosin by a kinase from chicken embryo.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

In vitro phosphorylation of tropomyosin by a kinase from chicken embryo.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider