Effects of nystatin‐mediated intracellular ion substitution on membrane currents in calf Purkinje fibres
Why the study?
Does nystatin-mediated intracellular ion substitution affect membrane currents in calf Purkinje fibres?
Population
Calf cardiac Purkinje fibres
Comparison
Nystatin-mediated intracellular ion substitution vs Untreated or K-loaded preparations
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Facilitates isolation of Ca currents in Purkinje fibre experiments; leaves open translation to human cardiac electrophysiology.
Structured PICO
Does nystatin-mediated intracellular ion substitution affect membrane currents in calf Purkinje fibres?
P
Population
Calf cardiac Purkinje fibres
I
Intervention
Nystatin-mediated intracellular ion substitution (exchange of caesium for intracellular potassium)
C
Comparator
Untreated or K-loaded preparations
O
Outcome
Membrane currents (transient outward current and inward Ca current)surrogate
Nystatin-mediated Cs loading in calf Purkinje fibres inhibits transient outward K+ currents, unmasking inward Ca2+ currents.
Cite This Study
Marbán et al. (1982) studied this question.