What is the clinical evidence from this study?

Study design: Other. Population: Pulmonary Arterial Hypertension (n=83). Intervention: Pulmonary Arterial Hypertension vs. Normal control. Primary outcome: Diagnostic value (AUC) of hub gene CCL5 (AUC 0.889, 95% CI 81.73%-96.07%).

What does this research mean for the field?

Integrated bioinformatic analysis of microarray data identifies 5 hub genes (CCL5, CXCL12, VCAM1, CXCR1, and SPP1) that are differentially expressed in pulmonary arterial hypertension and may serve as potential diagnostic biomarkers or therapeutic targets. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

January 1, 2021BioMed Research InternationalOpen Access

Screening of Hub Genes Associated with Pulmonary Arterial Hypertension by Integrated Bioinformatic Analysis

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Key result

Novel hub gene CCL5 shows strong diagnostic accuracy for pulmonary arterial hypertension with an AUC ~0.89.

AUC 0.889
95% CI 81.73%-96.07%
n=83

Why the study?

Pulmonary arterial hypertension has a low survival rate and an incompletely understood pathogenesis, requiring the identification of clinical molecular markers to support diagnosis and targeted treatments.

Population

Microarray dataset of PAH and normal control samples from GEO

Comparison

PAH samples vs normal control samples

Design

Integrated bioinformatic analysis of a microarray dataset

Authors

YZYu ZengSichuan Cancer Hospital NLNanhong LiSouthern Medical University ZZZhenzhen ZhengShanxi Agricultural University

Discussion

Member takes

Implication

May identify CCL5 as a PAH biomarker in preclinical data; leaves open prospective human validation.

Structured PICO

Population

83 lung tissue samples (58 with pulmonary arterial hypertension and 25 normal controls) analyzed via bioinformatics to identify diagnostic hub genes.

Exposure

Bioinformatic analysis including screening of differentially expressed genes (DEGs), functional enrichment analyses, protein-protein interaction (PPI) network construction, and miRNA-TF-hub gene network construction

Comparator

Normal lung tissue samples

Outcome

Identification of hub genes and evaluation of their diagnostic value using receiver operating characteristic (ROC) curvessurrogate

Main Result

Effect estimate: AUC 0.889 (95% CI 81.73%-96.07%)

Integrated bioinformatic analysis identified 5 hub genes (CCL5, CXCL12, VCAM1, CXCR1, and SPP1) that may serve as potential diagnostic biomarkers or therapeutic targets for pulmonary arterial hypertension.

Limitations

The sample size was relatively small.
The study was based on bioinformatics and computer analysis, requiring verification by in vivo and in vitro experiments.
small sample size
results based on computer analysis requiring in vivo and in vitro experimental verification

Cite This Study

Zeng et al. (2021) studied Pulmonary Arterial Hypertension (n=83). Pulmonary Arterial Hypertension vs. Normal control was evaluated on Diagnostic value (AUC) of hub gene CCL5 (AUC 0.889, 95% CI 81.73%-96.07%). Bioinformatic analysis identified five hub genes associated with pulmonary arterial hypertension, with CCL5 demonstrating an excellent diagnostic value (AUC 0.889).

synapsesocial.com/papers/6a1c04a0b33628da419d1e45 https://doi.org/10.1155/2021/6626094

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January 1, 2021BioMed Research InternationalOpen Access

Screening of Hub Genes Associated with Pulmonary Arterial Hypertension by Integrated Bioinformatic Analysis

View Full Paper

Ask AI

Bookmark

Key result

Novel hub gene CCL5 shows strong diagnostic accuracy for pulmonary arterial hypertension with an AUC ~0.89.

AUC 0.889
95% CI 81.73%-96.07%
n=83

Why the study?

Population

Microarray dataset of PAH and normal control samples from GEO

Comparison

PAH samples vs normal control samples

Design

Integrated bioinformatic analysis of a microarray dataset

Authors

YZYu ZengSichuan Cancer Hospital NLNanhong LiSouthern Medical University ZZZhenzhen ZhengShanxi Agricultural University

Discussion

Member takes

Implication

May identify CCL5 as a PAH biomarker in preclinical data; leaves open prospective human validation.

Structured PICO

Population

83 lung tissue samples (58 with pulmonary arterial hypertension and 25 normal controls) analyzed via bioinformatics to identify diagnostic hub genes.

Exposure

Comparator

Normal lung tissue samples

Outcome

Identification of hub genes and evaluation of their diagnostic value using receiver operating characteristic (ROC) curvessurrogate

Main Result

Effect estimate: AUC 0.889 (95% CI 81.73%-96.07%)

Limitations

The sample size was relatively small.
The study was based on bioinformatics and computer analysis, requiring verification by in vivo and in vitro experiments.
small sample size
results based on computer analysis requiring in vivo and in vitro experimental verification

Cite This Study

synapsesocial.com/papers/6a1c04a0b33628da419d1e45 https://doi.org/10.1155/2021/6626094

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