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April 11, 2008Human Mutation

A DNA resequencing array for pathogenic mutation detection in hypertrophic cardiomyopathy

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Why the study?

Does a custom DNA resequencing array effectively detect pathogenic mutations in patients with hypertrophic cardiomyopathy?

Population

38 unrelated patients with hypertrophic cardiomyopathy (HCM), including 17 familial and 21 sporadic cases.

Design

Cross-sectional

Key result

A custom DNA resequencing array achieved a 96.92% mean nucleotide call rate, identifying pathogenic mutations in 60% of familial and 10% of sporadic hypertrophic cardiomyopathy cases.

Authors

SFSiv FokstuenRLRobert LyleAMAnalia Muñoz

Discussion

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Overview

May support targeted screening in familial HCM; leaves open prospective validation in sporadic cases.

Study Design

Type

Observational (n=38)

Structured PICO

Does a custom DNA resequencing array effectively detect pathogenic mutations in patients with hypertrophic cardiomyopathy?

P
Population
38 unrelated patients with hypertrophic cardiomyopathy (HCM), including 17 familial and 21 sporadic cases.
I
Intervention
Custom DNA resequencing array targeting 12 genes implicated in HCM (MYH7, MYBPC3, TNNT2, TPM1, TNNI3, MYL3, MYL2, CSRP3, PLN, ACTC, TNNC1, and PRKAG2).
O
Outcome
Detection of pathogenic mutationssurrogate

A custom DNA resequencing array provides a rapid and effective method for detecting pathogenic mutations in patients with hypertrophic cardiomyopathy, particularly in familial cases.

Cite This Study

Fokstuen et al. (2008) conducted an observational in Hypertrophic cardiomyopathy (n=38). Custom DNA resequencing array was evaluated on Pathogenic mutation detection. A custom DNA resequencing array achieved a 96.92% mean nucleotide call rate, identifying pathogenic mutations in 60% of familial and 10% of sporadic hypertrophic cardiomyopathy cases.

synapsesocial.com/papers/6a1c6896973ffece4bc3c6efhttps://doi.org/10.1002/humu.20749
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Rapid detection of genetic variants in hypertrophic cardiomyopathy by custom DNA resequencing array in clinical practice2011 · 48 citations
  2. 2Array-Based Resequencing Assay for Mutations Causing Hypertrophic Cardiomyopathy2008 · 39 citations
  3. 3Mutation spectrum in a large cohort of unrelated consecutive patients with hypertrophic cardiomyopathy2003 · 228 citations
  4. 4Mutation Analysis of the Main Hypertrophic Cardiomyopathy Genes Using Multiplex Amplification and Semiconductor Next-Generation Sequencing2014 · 60 citations
  5. 5A Next-Generation Sequencing Approach to Identify Gene Mutations in Early- and Late-Onset Hypertrophic Cardiomyopathy Patients of an Italian Cohort2016 · 32 citations