Effects of intact and hydrolysed blue whiting proteins on blood pressure and markers of kidney function in obese Zucker fa/fa rats

Figures

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  Fig. 1. Increases in mean systolic blood pressure (a) and diastolic blood pressure (b) from baseline to end point. The figure shows values as the mean with their standard deviation shown by vertical bars for N = 5 rats in the control group, N = 6 rats in the BW-WM group, N = 6 rats in the BW-HA group and N = 6 rats in the BW-HP group. BW-WM group, BW-HA group, BW-HP group and control group were compared using one-way ANOVA. p ANOVA values were 0.031 and 0.047 for comparisons of systolic and diastolic blood pressure, respectively. Fisher’s LSD was used as post hoc test and different letters indicate significant differences between groups; p < 0.05 was considered significant; BW-WM blue whiting whole meal, BW-HA blue whiting protein hydrolysate prepared with Alcalase®, BW-HP blue whiting protein hydrolysate prepared with Protamex®, ANOVA analysis of variance, LSD least significant difference

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  Fig. 2. IC10 values for renin inhibition (a) and IC50 values for ACE inhibition (b) by dietary proteins. Data are presented as the amount of protein in ug/ml needed to inhibit 10% of renin activity and 50% of the ACE activity in a 0.25 U ACE assay, respectively. Data are presented as the mean with their standard error of mean shown by vertical bars for two different measurements. The dietary proteins were compared using one-way ANOVA. Casein was not included in the ANOVA analysis for renin inhibition since no measurable renin inhibition was detected for casein. Fisher’s LSD was used as post hoc test and different letters indicate significant differences between proteins; p < 0.05 was considered significant. Casein was prepared with trypsin; BW-WM blue whiting whole meal prepared with trypsin, BW-HA blue whiting protein hydrolysate prepared with Alcalase®, BW-HP blue whiting protein hydrolysate prepared with Protamex®, ANOVA analysis of variance, LSD least significant difference