Key result
Exposure of bovine aortic endothelial cells to a large negative stress phase angle (-180 deg) significantly decreased nitric oxide secretion at 5 hours compared to a 0 degree angle.
Why the study?
Does a large negative stress phase angle reduce nitric oxide production in bovine aortic endothelial cells compared to a stress phase angle of 0 degrees?
Does a large negative stress phase angle reduce nitric oxide production in bovine aortic endothelial cells compared to a stress phase angle of 0 degrees?
A large negative stress phase angle (-180 deg) significantly attenuates nitric oxide production in endothelial cells, supporting the hypothesis that complex hemodynamics play a role in pro-atherogenic vascular remodeling.
Answers are grounded in this paper and cite their sources.
Extends in vitro evidence linking hemodynamics to endothelial responses; hypothesis-generating for atherosclerosis mechanisms, requiring in vivo validation.
Hemodynamics plays an important role in cardiovascular physiology and pathology. Pulsatile flow (Q), pressure (P), and diameter (D) waveforms exert wall shear stress (WSS), normal stress, and circumferential strain (CS) on blood vessels. Most in vitro studies to date have focused on either WSS or CS but not their interaction. Recently, we have shown that concomitant WSS and CS affect EC biochemical response modulated by the temporal phase angle between WSS and CS (stress phase angle, SPA). Large negative SPA has been shown to occur in regions of the circulation where atherosclerosis and intimal hyperplasia are prevalent. Here, we report that nitric oxide (NO) biochemical secretion was significantly decreased in response to a large negative SPA of -180 deg with respect to an SPA of 0 degrees in bovine aortic endothelial cells (BAEC) at 5 h. A new hemodynamic simulator for the study of the physiologic SPA was used to provide the hemodynamic conditions of pro-atherogenic (SPA = -180 deg) and normopathic (SPA = 0 deg) states. The role of complex hemodynamics in vascular remodeling, homeostasis, and pathogenesis can be advanced by further assessment of the hypothesis that a large negative SPA is pro-atherogenic.
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Dancu et al. (2006) studied Atherosclerosis / Hemodynamics. Large negative stress phase angle (SPA = -180 deg) vs. Normopathic state (SPA = 0 deg) was evaluated on Nitric oxide (NO) biochemical secretion. Exposure of bovine aortic endothelial cells to a large negative stress phase angle (-180 deg) significantly decreased nitric oxide secretion at 5 hours compared to a 0 degree angle.
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