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October 1, 1994Journal of Biological ChemistryOpen Access

Independence of type I angiotensin II receptor endocytosis from G protein coupling and signal transduction.

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Why the study?

Does angiotensin II receptor endocytosis depend on G protein coupling and signal transduction in COS-7 cells expressing rat AT1a receptors?

Population

COS-7 cells transiently expressing mutant and wild type cDNAs for the rat AT1a receptor

Comparison

Expression of mutant AT1a receptors and… vs Wild type AT1a receptor and untreated cells

Design

Preclinical

Authors

LHLászló HunyadySemmelweis UniversityABAlbert J. BaukalNational Institutes of HealthTBTamás BallaNational Institutes of Health

Discussion

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Implication

AT1a receptor endocytosis proceeds without G protein coupling or PLC activation; leaves open in vivo relevance to human cardiovascular disease.

Structured PICO

Does angiotensin II receptor endocytosis depend on G protein coupling and signal transduction in COS-7 cells expressing rat AT1a receptors?

P
Population
COS-7 cells transiently expressing mutant and wild type cDNAs for the rat AT1a receptor
I
Intervention
Expression of mutant AT1a receptors (deletion mutants of the third cytoplasmic loop, point mutations of Asp74 including D74N, D74H, D74Y, and a combined mutant) and treatment with pertussis toxin
C
Comparator
Wild type AT1a receptor and untreated cells
O
Outcome
Receptor internalization kinetics and G protein coupling/inositol phosphate responsessurrogate

Endocytosis of the AT1 receptor is independent of agonist-activated signal transduction, indicating that receptor internalization and activation of phospholipase C have different structural requirements.

Cite This Study

Hunyady et al. (1994) studied this question.

synapsesocial.com/papers/6a1c8a060e2acd31eb2fdabehttps://doi.org/10.1016/s0021-9258(17)31462-x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1FATE OF [125I]ANGI0TENSIN II IN ADRENAL ZONA GLOMERULOSA CELLS1986 · 47 citations
  2. 2Serine- and threonine-rich domain regulates internalization of muscarinic cholinergic receptors.1993 · 110 citations
  3. 3Structure-function of the human insulin-like growth factor-I receptor: a discordance of somatotroph internalization and signaling.1993 · 12 citations
  4. 4Processing of Angiotensin II (A-II) and (Sar1Ala8)A-II by Cultured Bovine Adrenocortical Cells*1986 · 95 citations
  5. 5Multiple GTP-binding proteins participate in clathrin-coated vesicle-mediated endocytosis.1993 · 187 citations