What does this research mean for the field?

The intracellular translocation of the Galphaq/11 protein induced by AT1A receptor internalization is dependent on the receptor internalization process itself, but occurs independently of G protein coupling and signal transduction mechanisms. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

February 1, 2001Molecular EndocrinologyOpen Access

A Functional Enhanced Green Fluorescent Protein (EGFP)-Tagged Angiotensin II AT1AReceptor Recruits the Endogenous Gαq/11 Protein to the Membrane and Induces Its Specific Internalization Independently of Receptor- G Protein Coupling in HEK-293 Cells

View Full Paper

Ask AI

Bookmark

Population

HEK-293 cells expressing EGFP-tagged angiotensin II AT(1A) receptor

Comparison

Binding of different agonist or antagonist ligands vs Unstimulated state or different ligands/receptor…

Design

Preclinical

Authors

SMStéphanie Miserey‐LenkeiCentre National de la Recherche Scientifique ZLZsolt LenkeiInserm CPCharles ParnotCentre National de la Recherche Scientifique

Discussion

Member takes

Implication

Does not inform clinical use of ARBs; hypothesis-generating for AT1A trafficking mechanisms in cardiovascular models.

Structured PICO

Population

HEK-293 cells expressing EGFP-tagged angiotensin II AT(1A) receptor

Intervention

Binding of different agonist or antagonist ligands (peptide and nonpeptide)

Comparator

Unstimulated state or different ligands/receptor mutants (D(74)N and Delta329)

Outcome

Subcellular distribution, internalization, and trafficking of the AT(1A) receptor and Galphaq/11 proteinsurrogate

This basic science study demonstrates that Galphaq/11 protein cellular translocation depends on AT(1A) receptor internalization rather than G protein coupling and signal transduction.

Cite This Study

Miserey‐Lenkei et al. (2001) studied this question.

synapsesocial.com/papers/6a1eb53e94615786b59a73b7 https://doi.org/10.1210/mend.15.2.0600

View Full Paper

Ask AI

Bookmark

A Functional Enhanced Green Fluorescent Protein (EGFP)-Tagged Angiotensin II AT1AReceptor Recruits the Endogenous Gαq/11 Protein to the Membrane and Induces Its Specific Internalization Independently of Receptor- G Protein Coupling in HEK-293 Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

A Functional Enhanced Green Fluorescent Protein (EGFP)-Tagged Angiotensin II AT1AReceptor Recruits the Endogenous Gαq/11 Protein to the Membrane and Induces Its Specific Internalization Independently of Receptor- G Protein Coupling in HEK-293 Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider