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October 27, 2000Circulation Research

Functional Reconstitution of the Angiotensin II Type 2 Receptor and GiActivation

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Population

Cultured COS-7 cells expressing the recombinant angiotensin II type 2 receptor

Design

Preclinical

Authors

JHJakob Lerche HansenGSGuy ServantTBThomas Baranski

Discussion

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Overview

Provides new in vitro assay for AT2 pharmacology; leaves open any clinical role in cardiovascular disease.

Structured PICO

P
Population
Cultured COS-7 cells expressing the recombinant angiotensin II type 2 (AT2) receptor (urea-extracted membranes)
I
Intervention
Angiotensin II and related ligands (Ang III, Ang1-7, [Sar1,Ala8]Ang II, CPG42112)
O
Outcome
Activation of purified heterotrimeric G proteins (alpha_i, alpha_o, alpha_q, alpha_s) measured by radioactive GTPgammaS bindingsurrogate

The AT2 receptor is fully capable of activating Gi, providing a new in vitro assay to examine AT2 receptor pharmacology.

Cite This Study

Hansen et al. (2000) studied this question.

synapsesocial.com/papers/6a7c28a6050daa66a5df7edahttps://doi.org/10.1161/01.res.87.9.753
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Intracellular Third Loop Domain of Angiotensin II Type-2 Receptor1996 · 115 citations
  2. 2Pathophysiological Role of Angiotensin II Type 2 Receptor in Cardiovascular and Renal Diseases1998 · 479 citations
  3. 3The AT2 Receptor Selectively Associates with Giα2 and Giα3 in the Rat Fetus1996 · 130 citations
  4. 4Analysis of Functional Domains of Angiotensin II Type 2 Receptor Involved in Apoptosis1999 · 74 citations
  5. 5Signals from the AT2 (Angiotensin Type 2) Receptor of Angiotensin II Inhibit p21ras and Activate MAPK (Mitogen-Activated Protein Kinase) to Induce Morphological Neuronal Differentiation in NG108–15 Cells1999 · 106 citations