Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
July 1, 1995Molecular and Cellular EndocrinologyOpen Access

Characterization of a rat type 2 angiotensin II receptor stably expressed in 293 cells

View Full Paper
Ask AI
Bookmark
Share

Population

Human embryonic kidney 293 cells stably transfected with a cDNA clone for the rat type 2 angiotensin II…

Comparison

Angiotensin II stimulation and CGP42112A binding vs Whole rat fetus and transiently transfected…

Design

Preclinical

Authors

MMMasashi MukoyamaMHMasatsugu HoriuchiMNMasatoshi Nakajima

Discussion

Loading...

Member takes

Overview

Challenges assumptions of AT2 receptor G protein coupling; leaves open its mechanisms in native cardiovascular tissues.

Structured PICO

P
Population
Human embryonic kidney 293 cells stably transfected with a cDNA clone for the rat type 2 (AT2) angiotensin II receptor
I
Intervention
Angiotensin II stimulation and CGP42112A binding
C
Comparator
Whole rat fetus and transiently transfected COS-7 cells
O
Outcome
Receptor binding characteristics, G protein coupling, second messenger system activation, and receptor internalizationsurrogate

The rat AT2 receptor stably expressed in HEK 293 cells does not couple to major G protein signaling pathways or undergo agonist-stimulated internalization.

Cite This Study

Mukoyama et al. (1995) studied this question.

synapsesocial.com/papers/6a74ab718d2049c8dd033371https://doi.org/10.1016/0303-7207(95)03586-v
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular cloning of a novel angiotensin II receptor isoform involved in phosphotyrosine phosphatase inhibition.1993 · 716 citations
  2. 2Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors.1993 · 770 citations
  3. 3Purification and characterization of angiotensin II AT2 receptors from neonatal rat kidney.1993 · 15 citations
  4. 4Cloning, Expression and Regulation of Angiotensin II Receptors1994 · 21 citations
  5. 5Functional Reconstitution of the Angiotensin II Type 2 Receptor and G<sub>i</sub>Activation2000 · 70 citations