Inhibition of Baroreflex by Angiotensin II via Fos Expression in Nucleus Tractus Solitarii of the Rat

Sprague-Dawley rats anesthetized with pentobarbital sodium

Microinjection bilaterally of angiotensin II (5, 10, 20, or 40 pmol) into the nucleus tractus solitarii, with or without 15-mer antisense c-fos oligonucleotide, losartan (1.6 nmol), or PD 123,319 (1.6 nmol)

Control treatments with artificial cerebrospinal fluid, sense cDNA, or antisense oligonucleotide with a scrambled sequence

Spontaneous baroreceptor reflex response (magnitude of transfer function between systemic arterial pressure and heart rate signals) and Fos-like immunoreactivitysurrogate