Flavonoid-Rich Extract of Varthemia Iphionoides Increased Low-Density Lipoprotein Receptor and Decreased Hydroxymethylglutaryl Coa Reductase Expression in Hepg2 Cells

Varthemia iphionoides is a perennial herb that belongs to the Asteraceae family.In traditional medicine, it is used to alleviate abdominal pain, manage diabetes and aid in weight loss.This work aims to study the mechanism of its hypocholesterolaemia effect and identify the active constituents of its aerial parts.The hypocholesterolaemia effect of V. iphionoides extract was investigated in HepG2 cells by determining HMG-CoA reductase and low-density lipoprotein receptor (LDLR) gene expression using Q-PCR.The constituents of V. iphionoides extract were determined using a non-targeted profiling method using ultraperformance liquid chromatography coupled to a photodiode array.QTOF-MS was operated in positive ionisation mode.The half maximal inhibitory concentration (IC50) of V. iphionoides in HepG2 cells was 176.0 g/mL after 24 h.V. iphionoides extract (88 g/mL) significantly decreased HMG-CoA reductase gene expression, while 176 g/mL of its aerial parts extract increased LDLR gene expression after 24 h incubation.QTOF-MS analysis resulted in the identification of 11 flavonoids, two phenolic compounds, nicotinamide and phenylalanine.In conclusion, this study reports for the first time that V. iphionoides downregulates HMG-CoA reductase and upregulates the LDLR gene.The latter mechanism functions in a manner akin to statins. RezumatVarthemia iphionoides este o plant peren din familia Asteraceae.n medicina tradiional, este utilizat pentru ameliorarea durerilor abdominale, gestionarea diabetului i ajutarea la echilibrarea greutii.Aceast lucrare are ca scop studierea mecanismului efectului hipocolesterolemiant i identificarea constituenilor activi din prile aeriene ale plantei.Efectul hipocolesterolemiant al extractului de V. iphionoides a fost investigat n celulele HepG2 prin determinarea expresiei genelor pentru HMG-CoA reductaz i receptorul pentru lipoproteine cu densitate sczut (LDLR) folosind Q-PCR.Constituenii extractului de V. iphionoides au fost determinai folosind QTOF-MS.Concentraia inhibitorie medie (IC50) a V. iphionoides n celulele HepG2 a fost de 176,0 g/mL dup 24 de ore.Extractul de V. iphionoides (88 g/mL) a sczut semnificativ expresia genei HMG-CoA reductaz, n timp ce 176 g/mL din extractul prilor aeriene au crescut expresia genei LDLR dup 24 de ore de incubare.Analiza QTOF-MS a dus la identificarea a 11 flavonoide, 2 compui fenolici, nicotinamid i fenilalanin.n concluzie, acest studiu raporteaz pentru prima dat c V. iphionoides reduce expresia genei HMG-CoA reductaz i crete expresia genei LDLR.Acest mecanism funcioneaz similar cu statinele.