What question did this study set out to answer?

Investigate the intracellular signaling mechanisms, specifically the p38 MAPK and HSP27 pathway, that govern smooth muscle cell migration in response to growth factors and proinflammatory cytokines.

August 1, 1999Journal of Biological ChemistryOpen Access

A Role for p38MAPK/HSP27 Pathway in Smooth Muscle Cell Migration

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Authors

JHJason C. HedgesOregon Health & Science University MDMelissa A. DechertUniversity of Nevada, Reno IYIlia A. YambolievUniversity of Nevada, Reno

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Implication

In vitro study reveals that p38 MAPK and HSP27 signaling mediates migration in tracheal smooth muscle cells, suggesting a molecular therapeutic target for airway and vascular diseases.

Key Points

Investigate the intracellular signaling mechanisms, specifically the p38 MAPK and HSP27 pathway, that govern smooth muscle cell migration in response to growth factors and proinflammatory cytokines.
Assessed cell migration in cultured tracheal myocytes challenged with platelet-derived growth factor (PDGF), interleukin-1β (IL-1β), and transforming growth factor-β (TGF-β).
Evaluated pathway mechanics using the p38 MAPK chemical inhibitor SB203580 alongside phosphorylation time-course assays.
Employed adenoviral vectors to express constitutively active MKK6b(E), dominant-negative p38α MAPK, or an HSP27 phosphorylation mutant.
Stimulation with PDGF, IL-1β, and TGF-β induced p38 MAPK and HSP27 phosphorylation, whereas SB203580 blocked HSP27 phosphorylation and inhibited migration.
Overexpression of constitutively active upstream activator MKK6b(E) augmented smooth muscle cell migration.
Expression of dominant-negative p38α MAPK and an HSP27 phosphorylation mutant completely blocked cell migration.

Cite This Study

Hedges et al. (1999) studied this question.

synapsesocial.com/papers/6a1d372dba65f5ee325e02e2 https://doi.org/10.1074/jbc.274.34.24211

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Implication

In vitro study reveals that p38 MAPK and HSP27 signaling mediates migration in tracheal smooth muscle cells, suggesting a molecular therapeutic target for airway and vascular diseases.

A Role for p38MAPK/HSP27 Pathway in Smooth Muscle Cell Migration

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Key Points

Cite This Study

A Role for p38MAPK/HSP27 Pathway in Smooth Muscle Cell Migration

Discussion

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Implication

Key Points

Cite This Study

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