What question did this study set out to answer?

To examine whether the p38 MAP kinase pathway mediates morphological changes and cytoskeletal alignment in endothelial cells exposed to fluid shear stress.

January 1, 2001AJP Heart and Circulatory Physiology

Role of p38 MAP kinase in endothelial cell alignment induced by fluid shear stress

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Authors

NANobuyoshi AzumaAsahikawa Medical University NANobuyuki AkasakaMuroran Institute of Technology HKHiroyuki KitoChiba Cerebral and Cardiovascular Center

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Implication

In vitro study reveals that p38 MAP kinase signaling drives shear stress-induced alignment in endothelial cells, highlighting its essential role in vascular cytoskeletal remodeling.

Key Points

To examine whether the p38 MAP kinase pathway mediates morphological changes and cytoskeletal alignment in endothelial cells exposed to fluid shear stress.
Cultured bovine aortic endothelial cells were exposed to 14 dyn/cm² of laminar steady shear stress.
Cells were treated with the selective p38 inhibitor SB-203580 (1 µM) or transfected with a dominant-negative p38-alpha mutant to evaluate functional dependency.
Fluid shear stress stimulated peak activation of p38 (sixfold at 5 min), MAPKAP kinase 2 (sixfold at 5 min), and HSP25 (threefold at 30 min) relative to static control cells.
Treatment with SB-203580 prevented flow-induced cell orientation, yielding mean alignment angles of 17° without inhibitor, 50° with inhibitor, and 43° in static controls (P < 0.05).
Transfection with dominant-negative p38-alpha abolished stress fiber formation and induced random endothelial cell alignment despite exposure to shear stress.

Cite This Study

Azuma et al. (2001) studied this question.

synapsesocial.com/papers/6a906ddb6bfd95d7bd4de3db https://doi.org/10.1152/ajpheart.2001.280.1.h189

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Implication

In vitro study reveals that p38 MAP kinase signaling drives shear stress-induced alignment in endothelial cells, highlighting its essential role in vascular cytoskeletal remodeling.

Role of p38 MAP kinase in endothelial cell alignment induced by fluid shear stress

Discussion

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Implication

Key Points

Cite This Study

Role of p38 MAP kinase in endothelial cell alignment induced by fluid shear stress

Discussion

Member takes

Implication

Key Points

Cite This Study

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