What question did this study set out to answer?

To determine how varying levels of laminar shear stress influence endothelial cell elongation and alignment, and to evaluate the necessity of p38 MAPK in directing these morphological changes.

January 1, 2006Endothelium

p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress

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Authors

TKTakayuki KadohamaMuroran Institute of Technology NANobuyuki AkasakaMuroran Institute of Technology KNKengo NishimuraToyobo (Japan)

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Implication

In vitro study reveals that p38 MAPK activation drives fluid shear stress-induced alignment in endothelial cells, indicating a mechanotransduction threshold for vascular remodeling.

Key Points

To determine how varying levels of laminar shear stress influence endothelial cell elongation and alignment, and to evaluate the necessity of p38 MAPK in directing these morphological changes.
Cultured bovine aortic endothelial cells were subjected to laminar steady shear stress at 1, 4, 7, 14, or 20 dyne/cm² for 24 hours.
Morphometric parameters, specifically orientation angle and shape index, were quantified in the presence or absence of the p38 MAPK inhibitor SB-203580.
Shear stress progressively reduced the average orientation angle from 41° in static cells to 34°, 33°, 16°, 11°, and 10° at 1, 4, 7, 14, and 20 dyne/cm², respectively.
Shape index decreased from 0.76 under static conditions to 0.72, 0.72, 0.65, 0.50, and 0.47 across increasing shear levels, establishing a minimum threshold of 7 to 14 dyne/cm² for marked elongation and alignment.
Inhibition of p38 MAPK with SB-203580 completely prevented shear-induced cell orientation and retained a shape index similar to static controls.

Cite This Study

Kadohama et al. (2006) studied this question.

synapsesocial.com/papers/6a9524f195cf49f4209e71c3 https://doi.org/10.1080/10623320600660219

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Implication

In vitro study reveals that p38 MAPK activation drives fluid shear stress-induced alignment in endothelial cells, indicating a mechanotransduction threshold for vascular remodeling.

p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress

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Implication

Key Points

Cite This Study

p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress

Discussion

Member takes

Implication

Key Points

Cite This Study

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