What question did this study set out to answer?

The study investigates whether reduced glucose oxidation in diabetic hearts is due to biochemical changes or altered insulin levels.

April 1, 2000AJP Heart and Circulatory Physiology

Contribution of malonyl-CoA decarboxylase to the high fatty acid oxidation rates seen in the diabetic heart

Q: What does this research mean for the field?

Increased malonyl-CoA decarboxylase (MCD) activity, rather than circulating levels of substrates or insulin, contributes to the high fatty acid oxidation rates and reduced glucose oxidation rates seen in diabetic hearts. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

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Population

Isolated working hearts from control or diabetic rats

Comparison

Aerobic perfusion with either… vs Control rat hearts vs diabetic rat hearts…

Design

Preclinical

Authors

JSJun SakamotoTaisei (Japan)RBRick L. BarrSynlab Czech (Czechia)KKKatherine M. KavanaghUniversity of Calgary

Discussion

Member takes

Implication

May identify MCD as a therapeutic target to restore glucose oxidation in diabetic hearts; leaves open translation from rodent models.

Key Points

The study investigates whether reduced glucose oxidation in diabetic hearts is due to biochemical changes or altered insulin levels.
Isolated working hearts from control and diabetic rats were perfused with varying glucose and palmitate levels.
Glucose and fatty acid oxidation rates were measured under different conditions with insulin administration.
Activity of key enzymes (AMPK, ACC, MCD) involved in fatty acid oxidation was assessed in heart tissues.
In diabetic hearts, glucose oxidation was extremely low (9 nmol x g dry wt(-1) x min(-1)) and unresponsive to insulin.
Increased MCD activity and expression were found in diabetic hearts compared to controls, indicating a link to high fatty acid oxidation.
Control hearts showed increased glucose oxidation with insulin, but this effect did not translate to diabetic hearts.

Structured PICO

Population

Isolated working hearts from control or diabetic rats (streptozotocin, 55 mg/kg iv administered 6 wk before study)

Intervention

Aerobic perfusion with either low-fat/low-glucose buffer (5 mM [(14)C]glucose and 0.4 mM [(3)H]palmitate) or high-fat/high-glucose buffer (20 mM [(14)C]glucose and 1.2 mM [(3)H]palmitate) +/- 100 microU/ml insulin

Comparator

Control rat hearts vs diabetic rat hearts; different perfusion buffers

Outcome

Myocardial glucose oxidation and palmitate oxidation rates, and activity of AMPK, ACC, and MCDsurrogate

Increased malonyl-CoA decarboxylase (MCD) activity contributes to the high fatty acid oxidation rates and reduced glucose oxidation rates seen in diabetic rat hearts, independent of circulating substrate or insulin levels.

Cite This Study

Sakamoto et al. (2000) studied this question.

synapsesocial.com/papers/6a1d411eba3016ff712f53bf https://doi.org/10.1152/ajpheart.2000.278.4.h1196

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Contribution of malonyl-CoA decarboxylase to the high fatty acid oxidation rates seen in the diabetic heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Contribution of malonyl-CoA decarboxylase to the high fatty acid oxidation rates seen in the diabetic heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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