October 1, 1991AJP Heart and Circulatory Physiology
Effects of free fatty acids and dichloroacetate on isolated working diabetic rat heart
Key result
Perfusion with the glucose oxidation stimulator dichloroacetate (DCA) acutely reversed cardiac function depression in isolated working diabetic rat hearts.
Why the study?
Does dichloroacetate improve cardiac function in isolated working hearts from diabetic rats?
Population
Isolated working hearts from 6-week streptozotocin (STZ)-induced diabetic rats and age-matched controls
Comparison
Perfusion with 1.2 mM palmitate plus the glucose… vs Perfusion with 1.2 mM palmitate alone, and…
Design
Preclinical
Authors
Discussion
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Implication
DCA effects in diabetic rat hearts remain hypothesis-generating; should not inform clinical practice without human trials.
Key Points
- This research aims to investigate the effects of free fatty acids and dichloroacetate on cardiac function in isolated diabetic rat hearts.
- Compared control and diabetic isolated working rat hearts after 6-weeks of streptozotocin treatment (N=...).
- Assessed cardiac function via heart rate, heart rate peak systolic pressure product, left ventricular developed pressure, and rate of pressure rise.
- Administered palmitate and dichloroacetate in perfusate to evaluate their effects on heart function.
- Diabetic hearts exhibited reduced heart rate and cardiac function compared to controls.
- Palmitate addition did not enhance heart function but further depressed +dP/dt in diabetic hearts.
- Dichloroacetate improved cardiac function parameters in diabetic hearts to levels similar to controls.
Structured PICO
Does dichloroacetate improve cardiac function in isolated working hearts from diabetic rats?
P
Population
Isolated working rat hearts from 6-week streptozotocin-induced diabetic rats and age-matched controls.
I
Intervention
Perfusion with 1.2 mM palmitate plus the glucose oxidation stimulator dichloroacetate (DCA)
C
Comparator
Perfusion with 1.2 mM palmitate alone, and age-matched control hearts
O
Outcome
Cardiac function assessed by heart rate (HR), heart rate peak systolic pressure product (HR.PSP), left ventricular developed pressure (LVDP), and rate of pressure rise (+dP/dt)surrogate
Stimulation of glucose oxidation with dichloroacetate acutely reverses cardiac dysfunction in isolated diabetic rat hearts, highlighting the role of metabolic derangements in diabetic cardiomyopathy.
Cite This Study
Nicholl et al. (1991) studied Diabetes mellitus. Dichloroacetate (DCA) plus palmitate vs. Control hearts and diabetic hearts without DCA was evaluated on Cardiac function (HR, HR.PSP, LVDP, +dP/dt). Perfusion with the glucose oxidation stimulator dichloroacetate (DCA) acutely reversed cardiac function depression in isolated working diabetic rat hearts.