What question did this study set out to answer?

To investigate the effects of acute alloxan diabetes on cardiac performance in isolated rat hearts.

June 1, 1979AJP Heart and Circulatory Physiology

Cardiac performance of isolated perfused hearts from alloxan diabetic rats

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Why the study?

Does perfusion with insulin, high glucose, or octanoate improve cardiac performance in isolated hearts from acute alloxan diabetic rats compared to physiologic glucose alone?

Population

Isolated perfused working hearts from rats with acute alloxan diabetes (3 days)

Comparison

Perfusion with 5 mM glucose plus 10 M insulin… vs Perfusion with physiologic concentration of…

Design

Preclinical

Authors

TMThomas B. MillerCalifornia Institute of Technology

Discussion

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Implication

Substrate modulation may mitigate acute diabetic cardiac impairment in rats; leaves open translation to human diabetic cardiomyopathy.

Key Points

To investigate the effects of acute alloxan diabetes on cardiac performance in isolated rat hearts.
Used isolated perfused working hearts from alloxan diabetic rats (3 days post-diabetes)
Measured systolic pressure development and cardiac output while varying glucose and insulin levels
Analyzed glucose uptake and tissue ATP concentrations during perfusion with different substrates.
Heart function decreased with 40% reduction in glucose uptake, 20% reduction in ATP concentrations
Insulin and higher glucose concentrations improved systolic pressure and cardiac output
Diabetes-related cardiac impairment linked to inability to utilize glucose efficiently for energy production.

Structured PICO

Does perfusion with insulin, high glucose, or octanoate improve cardiac performance in isolated hearts from acute alloxan diabetic rats compared to physiologic glucose alone?

Population

Isolated perfused working hearts from rats with acute alloxan diabetes (3 days)

Intervention

Perfusion with 5 mM glucose plus 10(-8) M insulin, 10 or 30 mM glucose, or 1 mM octanoate

Comparator

Perfusion with physiologic concentration of glucose (5 mM) alone

Outcome

Cardiac performance (systolic pressure development and cardiac output)surrogate

The defect in cardiac performance associated with acute insulin deficiency in diabetic rats is due to an inability to utilize physiologic glucose, which can be reversed by insulin, higher glucose, or alternative substrates like octanoate.

Cite This Study

Thomas B. Miller (1979) studied this question.

synapsesocial.com/papers/6a72c09eb5c1fe5ca9e004f5 https://doi.org/10.1152/ajpheart.1979.236.6.h808

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Cardiac performance of isolated perfused hearts from alloxan diabetic rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Cardiac performance of isolated perfused hearts from alloxan diabetic rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider