What does this research mean for the field?

The highly conserved Arg-Gly-Asp (RGD) cell attachment motif, previously considered essential for viability, can become completely dispensable for normal viral replication in foot-and-mouth disease virus through short-term evolution. Novelty: ClaimNovelty.CONTRADICTORY. Consensus alignment: ConsensusAlignment.CHALLENGES_CONSENSUS.

June 24, 1997Proceedings of the National Academy of SciencesOpen Access

Evolution subverting essentiality: Dispensability of the cell attachment Arg-Gly-Asp motif in multiply passaged foot-and-mouth disease virus

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Population

Foot-and-mouth disease virus C-S8c1 and its multiply passaged derivative C-S8c1p100, propagated in BHK-21…

Comparison

Serial passage in cell culture and selection of… vs Parental FMDV C-S8c1 virus

Design

Preclinical

Authors

MMMiguel Ángel Martı́nezUniversitat Autònoma de Barcelona NVNúria VerdaguerInstitut de Biologia Molecular de Barcelona MMMauricio G. MateuCentro de Biología Molecular Severo Ochoa

Discussion

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Implication

RGD motif not required for FMDV replication; challenges consensus on its essential role in aphthovirus viability.

Structured PICO

Population

Foot-and-mouth disease virus (FMDV) C-S8c1 and its multiply passaged derivative C-S8c1p100, propagated in BHK-21 cell monolayers

Intervention

Serial passage in cell culture (100 times) and selection of mutants resistant to neutralizing monoclonal antibody SD6

Comparator

Parental FMDV C-S8c1 virus

Outcome

Viral replication capacity and frequency of mutants resistant to neutralization by mAb SD6surrogate

The highly conserved RGD cell attachment motif in FMDV can become dispensable upon short-term evolution in cell culture, demonstrating the phenotypic flexibility of RNA viruses.

Cite This Study

Martı́nez et al. (1997) studied this question.

synapsesocial.com/papers/6a1d75331c2cbcb15c5e57dd https://doi.org/10.1073/pnas.94.13.6798

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Evolution subverting essentiality: Dispensability of the cell attachment Arg-Gly-Asp motif in multiply passaged foot-and-mouth disease virus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Evolution subverting essentiality: Dispensability of the cell attachment Arg-Gly-Asp motif in multiply passaged foot-and-mouth disease virus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider