What does this research mean for the field?

The coxsackievirus and adenovirus receptor (CAR) is a common cellular receptor that mediates the attachment and infection of both coxsackie B viruses and adenoviruses 2 and 5. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To isolate and characterize the cellular receptor responsible for mediating infection by coxsackie B viruses and adenoviruses 2 and 5.

February 28, 1997Science

Isolation of a Common Receptor for Coxsackie B Viruses and Adenoviruses 2 and 5

View Full Paper

Ask AI

Bookmark

Population

Nonpermissive hamster cells

Comparison

Transfection with coxsackievirus and adenovirus… vs Non-transfected cells (implied)

Design

Preclinical

Authors

JBJeffrey M. BergelsonChildren's Hospital of Philadelphia JCJennifer CunninghamNewYork–Presbyterian Hospital GDGustavo DroguettRegeneron (United States)

Discussion

Member takes

Implication

CAR may enable targeted viral vectors; leaves open translation to human gene therapy.

Key Points

To isolate and characterize the cellular receptor responsible for mediating infection by coxsackie B viruses and adenoviruses 2 and 5.
Isolated a complementary DNA (cDNA) clone encoding the coxsackievirus and adenovirus receptor (CAR).
Transfected nonpermissive hamster cells with CAR cDNA to evaluate viral attachment, infection susceptibility, and adenovirus-mediated gene transfer.
Transfection of CAR cDNA into nonpermissive hamster cells rendered them fully susceptible to coxsackie B virus attachment and productive infection.
CAR-expressing hamster cells bound adenovirus in a viral fiber-dependent manner and exhibited a 100-fold increase in susceptibility to adenovirus-mediated gene transfer.

Structured PICO

Population

Nonpermissive hamster cells

Intervention

Transfection with coxsackievirus and adenovirus receptor (CAR) complementary DNA

Comparator

Non-transfected cells (implied)

Outcome

Susceptibility to coxsackie B virus attachment and infection, and adenovirus-mediated gene transfer

The identification of CAR as a common receptor for coxsackie B viruses and adenoviruses provides insight into viral pathogenesis and has implications for therapeutic gene delivery.

Cite This Study

Bergelson et al. (1997) studied this question.

synapsesocial.com/papers/6a1d75331c2cbcb15c5e57df https://doi.org/10.1126/science.275.5304.1320

View Full Paper

Ask AI

Bookmark

February 28, 1997Science

Isolation of a Common Receptor for Coxsackie B Viruses and Adenoviruses 2 and 5

View Full Paper

Ask AI

Bookmark

Population

Nonpermissive hamster cells

Comparison

Transfection with coxsackievirus and adenovirus… vs Non-transfected cells (implied)

Design

Preclinical

Authors

JBJeffrey M. BergelsonChildren's Hospital of Philadelphia JCJennifer CunninghamNewYork–Presbyterian Hospital GDGustavo DroguettRegeneron (United States)

Discussion

Member takes

Implication

CAR may enable targeted viral vectors; leaves open translation to human gene therapy.

Key Points

To isolate and characterize the cellular receptor responsible for mediating infection by coxsackie B viruses and adenoviruses 2 and 5.
Isolated a complementary DNA (cDNA) clone encoding the coxsackievirus and adenovirus receptor (CAR).
Transfected nonpermissive hamster cells with CAR cDNA to evaluate viral attachment, infection susceptibility, and adenovirus-mediated gene transfer.
Transfection of CAR cDNA into nonpermissive hamster cells rendered them fully susceptible to coxsackie B virus attachment and productive infection.
CAR-expressing hamster cells bound adenovirus in a viral fiber-dependent manner and exhibited a 100-fold increase in susceptibility to adenovirus-mediated gene transfer.

Structured PICO

Population

Nonpermissive hamster cells

Intervention

Transfection with coxsackievirus and adenovirus receptor (CAR) complementary DNA

Comparator

Non-transfected cells (implied)

Outcome

Susceptibility to coxsackie B virus attachment and infection, and adenovirus-mediated gene transfer

The identification of CAR as a common receptor for coxsackie B viruses and adenoviruses provides insight into viral pathogenesis and has implications for therapeutic gene delivery.

Cite This Study

Bergelson et al. (1997) studied this question.

synapsesocial.com/papers/6a1d75331c2cbcb15c5e57df https://doi.org/10.1126/science.275.5304.1320

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark