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October 15, 1978Biochemical JournalOpen Access

Inhibition of adenylate cyclase by adenosine analogues in preparations of broken and intact human platelets. Evidence for the unidirectional control of platelet function by cyclic AMP

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Population

Preparations of broken and intact human platelets

Comparison

Adenosine analogues (including 9-adenine [SQ… vs Control conditions

Design

Preclinical

Authors

RHRichard J. HaslamSaint Joseph's UniversityMDM M L DavidsonMcMaster UniversityJDJohanne DesjardinsRoyal Victoria Regional Health Centre

Discussion

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Implication

Highlights cAMP mediation of PGE1 effects in models; leaves open human translation and therapeutic targeting.

Key Points

  • The study aims to explore how adenosine analogues affect adenylate cyclase activity and platelet function.
  • Analyzed the effects of various adenosine analogues on adenylate cyclase activity in human platelets.
  • Kinetic studies assessed non-competitive inhibition and co-operativity of selected compounds.
  • Investigated the impact of different compounds on platelet aggregation induced by ADP and vasopressin.
  • 2',5'-dideoxyadenosine and SQ 22536 caused 50% inhibition at 4 microns and 13 microns, respectively.
  • Both compounds decreased prostaglandin E1's inhibitory effects on platelet aggregation induced by ADP and vasopressin.
  • 2'-deoxyadenosine 3'-monophosphate did not affect platelet aggregation, indicating its specific action site.

Structured PICO

P
Population
Preparations of broken and intact human platelets
I
Intervention
Adenosine analogues (including 9-(tetrahydro-2-furyl)adenine [SQ 22536], 2',5'-dideoxyadenosine, and 2'-deoxyadenosine 3'-monophosphate)
C
Comparator
Control conditions (absence of analogues or presence of prostaglandin E1 alone)
O
Outcome
Adenylate cyclase activity and platelet aggregationsurrogate

The study demonstrates that cyclic AMP mediates the inhibitory effects of prostaglandin E1 on platelet aggregation but plays no significant role in resting platelet responses to aggregating agents.

Cite This Study

Haslam et al. (1978) studied this question.

synapsesocial.com/papers/6a1db563c8f6dd20ef4db0bchttps://doi.org/10.1042/bj1760083
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Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Cyclic 3′,5′-adenosine monophosphate in human blood platelets1971 · 196 citations
  2. 2Isolation of a unique peptide inhibitor of hormone-responsive adenylate cyclase.1975 · 25 citations