What question did this study set out to answer?

To determine whether exogenous apolipoprotein E-3 optimizes the cellular metabolism of normolipidemic human apo B-100 lipoproteins in cultured human fibroblasts.

September 1, 1988Arteriosclerosis An Official Journal of the American Heart Association IncOpen Access

Enhanced metabolism of normolipidemic human plasma very low density lipoprotein in cultured cells by exogenous apolipoprotein E-3.

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Authors

SES EisenbergHadassah Medical Center GFG. FriedmanSeattle Pacific University TVThomas VogelLos Alamos National Laboratory

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Implication

Experimental study demonstrates that exogenous apolipoprotein E-3 enhances VLDL clearance in cultured human fibroblasts, indicating its essential role in apo B-100 lipoprotein catabolism.

Key Points

To determine whether exogenous apolipoprotein E-3 optimizes the cellular metabolism of normolipidemic human apo B-100 lipoproteins in cultured human fibroblasts.
Isolated VLDL subfractions I, II, and III, as well as LDL, from five normolipidemic human subjects (one with phenotype E 4/3 and four with E 3/3).
Incubated cultured normal and receptor-negative human fibroblasts with 125I-labeled lipoproteins supplemented with recombinant or plasmatic apo E-3 or apo E-2.
Assessed lipoprotein binding, internalization, degradation, particle association, and down-regulation of cellular sterol synthesis across varying apo E-3 concentrations.
Metabolism (binding, cell association, and degradation) of VLDL fractions I, II, and III was negligible in the absence of apo E-3, whereas exogenous apo E-3 induced a many-fold enhancement across all three VLDL fractions without affecting LDL.
Optimal stimulation occurred at 4 to 6 µg apo E-3 per 15 µg VLDL-protein, leading to maximal particle binding and enhanced down-regulation of cellular sterol synthesis; these effects were not observed with apo E-2 or in receptor-negative cells.

Cite This Study

Eisenberg et al. (1988) studied this question.

synapsesocial.com/papers/6a1dd08049e88a0d41602c14 https://doi.org/10.1161/01.atv.8.5.480

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Implication

Experimental study demonstrates that exogenous apolipoprotein E-3 enhances VLDL clearance in cultured human fibroblasts, indicating its essential role in apo B-100 lipoprotein catabolism.

Enhanced metabolism of normolipidemic human plasma very low density lipoprotein in cultured cells by exogenous apolipoprotein E-3.

Discussion

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Implication

Key Points

Cite This Study

Enhanced metabolism of normolipidemic human plasma very low density lipoprotein in cultured cells by exogenous apolipoprotein E-3.

Discussion

Member takes

Implication

Key Points

Cite This Study

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