Molecular adaptation of cellular defences following preconditioning of the heart by repeated ischaemia

Isolated buffer-perfused rat hearts

Repeated ischemia/reperfusion (4 x PC: 5 min ischemia followed by 10 min reperfusion repeated four times, then 60 min reperfusion)

1 x PC (5 min ischemia followed by 10 min reperfusion, then 60 min reperfusion) and Control (buffer perfusion without ischemia/reperfusion)

Expression of stress-related and antioxidative genes (c-fos, c-myc, HSP 27, HSP 70, HSP 89, Mn-SOD, catalase) and proteins, and activities of antioxidative enzymessurrogate