What question did this study set out to answer?

To study the presence of connectin-like high molecular weight proteins in various vertebrate and invertebrate muscles.

April 1, 1986The Journal of Biochemistry

Sodium Dodecyl Sulfate Gel Electrophoresis Studies of Connectin-Like High Molecular Weight Proteins of Various Types of Vertebrate and Invertebrate Muscles1

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Population

Various types of muscles of vertebrates and invertebrates

Design

Preclinical

Authors

DHDi HuFirst Affiliated Hospital of Guangzhou Medical University SKSumiko KimuraJikei University School of Medicine KMKosçak MaruyamaChiba University

Discussion

Member takes

Implication

No immediate clinical implications for cardiac elasticity; leaves open connectin evolution and function across muscle types.

Key Points

To study the presence of connectin-like high molecular weight proteins in various vertebrate and invertebrate muscles.
SDS gel electrophoresis method used to extract and separate proteins from muscle tissues.
Analysis included skeletal and cardiac muscles from various vertebrates, as well as muscle samples from invertebrates like nematodes and arthropods.
Comparison of protein bands across different muscle types and species.
Connectin was detected in striated muscles of vertebrates like rabbits, chickens, and fish.
Connectin-like proteins present in certain invertebrate muscles (e.g., claw muscles of crabs) but absent in others (e.g., tail muscles of crayfish).
Smaller proteins were observed faintly in molluscan adductor muscles.

Structured PICO

Population

Various types of muscles of vertebrates (rabbit, chicken, turtle, snake, newt, frog, fish) and invertebrates (Amphioxus, C. elegans, earthworm, molluscan adductor muscles, arthropods)

Intervention

SDS gel electrophoresis method

Outcome

Detection of connectin or connectin-like high molecular weight proteinssurrogate

The study reveals that connectin is an elastic protein present in vertebrate striated (skeletal and cardiac) muscles, with variable presence in invertebrates.

Cite This Study

Hu et al. (1986) studied this question.

synapsesocial.com/papers/6a1e87f57f6e8bbb23690c71 https://doi.org/10.1093/oxfordjournals.jbchem.a135618

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Sodium Dodecyl Sulfate Gel Electrophoresis Studies of Connectin-Like High Molecular Weight Proteins of Various Types of Vertebrate and Invertebrate Muscles1

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Sodium Dodecyl Sulfate Gel Electrophoresis Studies of Connectin-Like High Molecular Weight Proteins of Various Types of Vertebrate and Invertebrate Muscles1

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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