What question did this study set out to answer?

This research aims to understand how H+ and Ca++ influence junctional conductance in cardiac myocytes.

June 1, 1990The Journal of General PhysiologyOpen Access

Gap junctional conductance between pairs of ventricular myocytes is modulated synergistically by H+ and Ca++.

Q: What is the clinical evidence from this study?

Study design: Other. Intervention: Acidification with CO2 and varying calcium levels vs. Air-equilibrated medium / low calcium medium. Primary outcome: Gap junctional conductance (gj).

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Key result

Gap junctional conductance between ventricular myocyte pairs is synergistically decreased by increased intracellular H+ and Ca++ concentrations.

Population

Cardiac ventricular myocyte pairs

Comparison

Sarcoplasmic acidification with CO2 under… vs Air-equilibrated medium (baseline conditions)

Design

Preclinical

Authors

RWRoy L. WhiteTemple University JDJeannette E. DoellerUniversity of Alabama at Birmingham VVVytas K. VerselisAlbert Einstein College of Medicine

Discussion

Member takes

Implication

Hypothesis-generating for ischemic conduction slowing; leaves open in vivo relevance and clinical translation.

Key Points

This research aims to understand how H+ and Ca++ influence junctional conductance in cardiac myocytes.
Measured gap junctional conductance between ventricular myocyte pairs in varying pH and calcium levels.
Utilized fura-2 fluorescence to assess intracellular calcium concentration.
Employed ion-selective microelectrodes to measure intracellular pH.
Gap junctional conductance significantly decreased with acidification (pH 6.0) and physiological calcium levels, presenting specific rates.
At low calcium, gj was minimally affected by acidification, suggesting a synergistic effect with calcium increase.
Calcium ionophore A23187 also reduced gj to near zero in acidic conditions, indicating a key interaction between H+ and Ca++.

Structured PICO

Population

Cardiac ventricular myocyte pairs

Intervention

Sarcoplasmic acidification with CO2 under varying extracellular and intracellular calcium conditions

Comparator

Air-equilibrated medium (baseline conditions)

Outcome

Gap junctional conductance (gj)surrogate

H+ and Ca++ act synergistically to decrease gap junctional conductance in ventricular myocytes, providing mechanistic insight into cellular uncoupling.

Cite This Study

White et al. (1990) studied this question. Acidification with CO2 and varying calcium levels vs. Air-equilibrated medium / low calcium medium was evaluated on Gap junctional conductance (gj). Gap junctional conductance between ventricular myocyte pairs is synergistically decreased by increased intracellular H+ and Ca++ concentrations.

synapsesocial.com/papers/6a1ea7ab6540130b7faf2425 https://doi.org/10.1085/jgp.95.6.1061

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Gap junctional conductance between pairs of ventricular myocytes is modulated synergistically by H+ and Ca++.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Gap junctional conductance between pairs of ventricular myocytes is modulated synergistically by H+ and Ca++.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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