What question did this study set out to answer?

To determine whether tissue lactate accumulation and lactate efflux in contracting red skeletal muscle are governed by intracellular oxygen availability.

August 1, 1986Journal of Applied Physiology

Lactate efflux is unrelated to intracellular PO2 in a working red muscle in situ

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Authors

RCR. J. ConnettUniversity of Rochester TGT. E. GayeskiUniversity of Alabama at Birmingham CHC. R. HonigUniversity of Illinois Urbana-Champaign

Discussion

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Implication

Experimental study demonstrates lactate release occurs independently of intracellular oxygenation in canine red muscle, indicating that anaerobic thresholds do not reflect muscle hypoxia.

Key Points

To determine whether tissue lactate accumulation and lactate efflux in contracting red skeletal muscle are governed by intracellular oxygen availability.
Assessed blood flow, lactate extraction, and tissue lactate in autoperfused canine gracilis muscles frozen in situ during twitch contractions at 1–8 Hz (10–100% VO2max).
Calculated intracellular PO2 (Pto2) via subcellular spectrophotometric determination of myoglobin saturation across more than 2,000 cells.
Tissue lactate correlated with VO2 but not with Pto2, and lactate efflux increased sharply above approximately 50% VO2max without a direct link to Pto2 or linear correlation to tissue lactate.
Pto2 exceeded the critical minimum PO2 for maximal VO2 in all 2,000 cells examined at 1–6 Hz, and localized anoxia observed in 3 muscles at 8 Hz did not result in greater lactate efflux compared to 6 non-anoxic muscles.

Cite This Study

Connett et al. (1986) studied this question.

synapsesocial.com/papers/6a1f1a494d2ebb160c7298cb https://doi.org/10.1152/jappl.1986.61.2.402

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August 1, 1986Journal of Applied Physiology

Lactate efflux is unrelated to intracellular PO2 in a working red muscle in situ

View Full Paper

Ask AI

Bookmark

Authors

RCR. J. ConnettUniversity of Rochester TGT. E. GayeskiUniversity of Alabama at Birmingham CHC. R. HonigUniversity of Illinois Urbana-Champaign

Discussion

Member takes

Implication

Experimental study demonstrates lactate release occurs independently of intracellular oxygenation in canine red muscle, indicating that anaerobic thresholds do not reflect muscle hypoxia.

Key Points

To determine whether tissue lactate accumulation and lactate efflux in contracting red skeletal muscle are governed by intracellular oxygen availability.
Assessed blood flow, lactate extraction, and tissue lactate in autoperfused canine gracilis muscles frozen in situ during twitch contractions at 1–8 Hz (10–100% VO2max).
Calculated intracellular PO2 (Pto2) via subcellular spectrophotometric determination of myoglobin saturation across more than 2,000 cells.
Tissue lactate correlated with VO2 but not with Pto2, and lactate efflux increased sharply above approximately 50% VO2max without a direct link to Pto2 or linear correlation to tissue lactate.
Pto2 exceeded the critical minimum PO2 for maximal VO2 in all 2,000 cells examined at 1–6 Hz, and localized anoxia observed in 3 muscles at 8 Hz did not result in greater lactate efflux compared to 6 non-anoxic muscles.

Cite This Study

Connett et al. (1986) studied this question.

synapsesocial.com/papers/6a1f1a494d2ebb160c7298cb https://doi.org/10.1152/jappl.1986.61.2.402

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