July 22, 2015Scientific ReportsOpen Access

CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators

Q: What is the clinical evidence from this study?

Study design: Observational. Population: Obesity (n=9). Intervention: Visceral Adipose Tissue (VAT) vs. Subcutaneous Adipose Tissue (SAT). Primary outcome: Differential protein secretion between VAT and SAT.

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Key result

Visceral and subcutaneous adipose tissues from obese patients exhibit distinct secretory profiles, with visceral fat over-secreting proteins related to immune response and extracellular matrix remodeling.

Population

Human visceral and subcutaneous adipose tissue explants from 3 non-diabetic obese patients with metabolic…

Comparison

Comparison of Isotope-Labeled Amino acid… vs Visceral adipose tissue versus Subcutaneous…

Design

Preclinical

Authors

ARArturo Roca-RivadaUniversité Libre de Bruxelles SBSusana B. BravoComplejo Hospitalario Universitario de Santiago DPDiego Pérez-SoteloInstituto de Investigación Sanitaria de Santiago

Discussion

Member takes

Implication

Hypothesis-generating for depot-specific inflammation in obesity; leaves open causal links and clinical relevance in humans.

Study Design

Type

Observational (n=9)

Structured PICO

Population

9 individuals, including 6 non-diabetic obese patients and 3 healthy subjects, who provided visceral and subcutaneous adipose tissue samples during abdominal surgery.

Exposure

Comparison of Isotope-Labeled Amino acid Incorporation Rates (CILAIR) using double isotope-labeling ([13C6]-Lys and [13C6,15N4]-Arg)

Comparator

Visceral adipose tissue (VAT) versus Subcutaneous adipose tissue (SAT)

Outcome

Identification and differential quantification of location-specific secreted proteinssurrogate

Visceral and subcutaneous adipose tissues in obese patients exhibit distinct secretome profiles, particularly regarding extracellular matrix remodeling and inflammation, reinforcing their differential contributions to metabolic disease.

Limitations

Impossibility to normalize the number of cells for each adipose depot
Culturing adipocytes and all components of the stromal-vascular fraction together makes discerning exact cell type contributions impracticable
Validation by western blot in whole secretome includes labeled and non-labeled contaminant proteins
Limited label incorporation time (72 h) may have missed important secreted adipokines like adiponectin and leptin
Small sample size (n=3 for main analysis)
Labeling incubation time is very limited in the CILAIR approach

Cite This Study

Roca-Rivada et al. (2015) conducted an observational in Obesity (n=9). Visceral Adipose Tissue (VAT) vs. Subcutaneous Adipose Tissue (SAT) was evaluated on Differential protein secretion between VAT and SAT. Visceral and subcutaneous adipose tissues from obese patients exhibit distinct secretory profiles, with visceral fat over-secreting proteins related to immune response and extracellular matrix remodeling.

synapsesocial.com/papers/6a1f2243e800cc4eef5532f3 https://doi.org/10.1038/srep12214

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CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators

Discussion

Member takes

Implication

Study Design

Structured PICO

Limitations

Cite This Study

CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators

Discussion

Member takes

Implication

Study Design

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider